When goat primordial germ cells were cultured on different feeder cells,MEF,GEF and BEF all could sustain goat primary embryonic germ cells,and MEF was finer than others.

• 以小鼠胎儿成纤维细胞(MEF)、山羊胎儿成纤维细胞(GEF)、牛胎儿成纤维细胞(BEF)为饲养层,在不添加细胞因子情况下,都可以培养出原代山羊的胚胎生殖细胞。