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- LCP were separated into three fractions (LCP1, LCP2 and LCP3) by anion exchange chromatography DEAE-Sepharose FF. 粗多糖LCP经DE-AE-SepharoseFF离子交换柱分离得到三个组分(LCP1、LCP2和LCP3);主要组分LCP1经高效体积排阻色谱和毛细管电泳鉴定为均一组分;分子量为3.;27×106Da。
- Ion exchange chromatography showed that all three capsules eluted as one major and several small peaks. 离子交换色谱显示,3种荚膜的活性部分均有一蛋白主峰和若干个小蛋白峰,主峰由蛋白和多糖构成,其比值分别为8.9:1,9.0:1,6.5:1。
- Thin layer chromatography(TLC)behavior of 14 different amino acids were studied in copper-tartrate medium by Ligand Exchange Chromatography(LEC). 本文应用配体交换色谱法(LEC)研究了14种氨基酸在 Cu~(2+)-酒石酸介质中的薄层色谱行为.
- Amino acid analysis was performed using ion exchange chromatography and was in the range of the standard reference for FAO/WHO. 氨基酸分析表明该产品符合FAO/WHO的参照标准。
- A review with 64 references is presented on eluents for non\|suppressed ion exchange chromatography with conductometric detection. 分别对非抑制型电导检测阴离子交换色谱法和阳离子交换色谱法的淋洗液进行了综述,引用文献64篇。
- A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated. 采用超滤-离子交换色谱分步洗脱法,对牛初乳中的乳过氧化物酶进行了分离和纯化。
- CEN Ag and corneal epithelial antigen(CEP Ag) were purified by anion exchange chromatography and affinity chromatography with 2B4.14.1 monoclonal antibody. 经离子交换层析及 2B4 .;14
- The product of pG2/Rosetta(DE3) was proved soluble,then it was purified to be electrophoretically pure by ammonium sulfate precipitationand cation exchange chromatography. pcGH2/Roseta(DE3)的表达产物为可溶性蛋白; 该产物用硫酸铵分级沉降和阳离子交换层析分离纯化,达到电泳纯。
- Acetone extractives in masson pine (Pinus massoniana lamb)groundwood pulp were isolated and analyzed through the employment of DEAE-Sephadex ion exchange chromatography and GC/MS. 利用DEAE-Sephadex离子交换色谱和气相色谱-质谱联用技术,对马尾松磨木浆丙酮抽出物的化学成分进行了分离及分析。
- In this paper the isolation and identification of trypsin inhibitors from porcine pancreas by CM-and DEAE-Cellulose ion exchange chromatography have been reported. 本文报告用CM和DEAE纤维素离子交换层析从猪胰脏分离出对胰蛋白酶有特异性抑制作用的抑制剂。
- Abstract: Among the procedure of tobramycin production, hydrolysis of the fermantation solution, purification by ion exchange chromatography and salt formation has been studied. 摘 要: 对硫酸妥布霉素生产中发酵液的水解、离子交换层离、成盐等条件进行了研究。
- Moreover, as the radial flow column in ion exchange chromatography was employed, the procedure was easy to be scaled up to the industrial production of r-SAK. 蛋白质回收率和活性回收率均为40%25以上,由于选用径向色谱柱,色谱参数可直接放大至工业化生产。
- A rapid method for the determination of thiabendazole was developed in six kind of Juice concentrate by ion exchange chromatography(IEC) and fluorescence detection. 建立离子交换色谱-荧光检测法快速测定6种浓缩果汁中噻菌灵残留量的分析方法。
- RSPS 1-3, which was a relative homogeneous fraction, was obtained after two different chromatography techniques, which were ion exchange chromatography and Sephadex chromatography. 通过离子交换和葡聚糖凝胶二级柱层析纯化方法获得菜籽多糖相对均一的组分RSPS1-3。
- Objective The gradient procedure was developed based on AOAC 2001.02 to determine galactooligosaccharide (soluble dietary fiber with low molecular weight) by high performance ion exchange chromatography with pulsed amperometric detection. 目的在AOAC2001·02方法基础上,优化淋洗液梯度,以高效离子色谱-脉冲安培检测法测定小分子可溶性膳食纤维低聚半乳糖。
- Objective: Get the pure EPF from the pregnancy sera through the ion exchange chromatography, affinity chromatography and reverse phage High Performance Liquid Chromatography(HPLC). 目的:采用离子交换层析、亲和层析和反相高效液相色谱(HPLC,High Performance Liquid Chromatography)技术,从人早孕血清中分离纯化早孕因子(EPF,Early Pregnancy Factor)。
- A thrombin-like enzyme (TLE) was isolated and purified from the venom of Agkistrodon acutus by ion exchange chromatography on DEAE-Sephadex A-50 and gel filtration on Sephadex G-75.The TLE(1.5U/kg) was given intravenously to anesthetized dogs . 本文借助 DEAE-sephadex A-50和 Sephadex G-75柱层析从尖吻蝮蛇毒(AAV)内分离并纯化出一种类凝血酶(TLE).
- A trypsin like enzyme(AGP, Mr 14kD) was purified after ammonium sulfate precipitation, Sephadex G 75 gel filtration, Sephadex DEAE A 25 ion exchange chromatography and SBBI Sepharose 4B affinity chromatography. 肠液经硫铵沉淀,SephadexG?75凝胶过滤,SephadexDEAEA?25离子交换和SBBI?Sepharose4B亲和层析,分离纯化出分子量约为14kD的巨尾阿丽蝇蛋白酶。
- Set up a method of isolation and purification EPF through ion exchange chromatography, affinity chromatography and reverse phage HPLC. Get 1.17mg high active EPF from 730ml pregnancy sera . 本课题建立了一条通过离子交换层析、亲和层析和反相HPLC从人早孕血清中分离纯化EPF的技术路线,并成功从730ml人早孕血清中分离得到1.;17mg活性较强的EPF。
- Based on the property of bacterial endotoxin,the effects of affinity chromatography,ion exchange chromatography and hydrophobic interaction chromatography on removal of endotoxin in the pu- rification of recombinant12 5Ser- IL - 2 were compared. 针对细菌内毒素的化学性质 ,比较了用亲和层析法、离子交换层析法和疏水相互作用层析法等 3种层析法去除基因重组 1 2 5Ser- IL- 2产品中细菌内毒素的效果。
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries