Set up a method of isolation and purification EPF through ion exchange chromatography, affinity chromatography and reverse phage HPLC. Get 1.17mg high active EPF from 730ml pregnancy sera .

• 本课题建立了一条通过离子交换层析、亲和层析和反相HPLC从人早孕血清中分离纯化EPF的技术路线，并成功从730ml人早孕血清中分离得到1.；17mg活性较强的EPF。