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- Methods:Messenger RNA expressions of nm23-H1 and nm23-H2 genewere investigated in 12 cases of human bladder transitional cell tumor and 6 cases of human normalbladder tissue by using the Northern blot techniques. 方法:應用Northernblot技術分析12例膀胱移行細胞癌和6例肉眼未見異常的癌旁膀胱粘膜nm23-H1和nm23-H2基因的表達。
- Southern blot technique developed to identify DNA fragments. 印跡雜交被用來發現DNA片段。
- Northern blot showed that PBP-Harm and GOBP2-Harm are specifically expressed in the antenna of H.armigera. Northern雜交結果表明:PBP-Harm和GOBP2-Harm都在棉鈴蟲觸角中特異性表達。
- The mRNA expression of ubiquitin in rat EDL muscle was determined by northern blot analysis. 應用northernblot方法測定伸指長肌組織泛素mRNA的表達變化 ,分析骨骼肌蛋白降解與泛素mRNA表達的關係。
- PAI-1 protein secretion by mesangial cells was measured by Wes tern blot and PAI-1 mRNA by both RT-PCR and Northern blot. RT-PCR和Northern雜交檢測PAI-1mRNA表達;
- ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 採用Dotblot雜交技術定量研究3組胃粘膜ET?1、NOSmRNA表達。
- With RT-PCR and Northern blot methods, the expression patterns of mNTKL-BPl were observed in the normal tissues of mouse. 用RT-PCR和Northern blot方法觀察到mNTKL-BP1基因在小鼠的正常組織中均有表達。
- Transgenic potato plants 1M, 2M, 3M, 5M, 10M, llM provided by laboratory were analyzed by Southern blot and Northern blot. ELISA檢測轉基因馬鈴薯病毒抗性數據顯示,溫室條件下,3個轉基因馬鈴薯株系(2M、3M、11M)具有病毒抗性,3個株系(1M、5M、10M)感病;
- Northern blot -- An electroblotting method in which RNA is transferred to a filter and detected by hybridization to32 P-labelled RNA or DNA. 北方轉漬法--一種電子轉印方法,將RNA轉移至濾紙上,加上要試驗的放射性標誌RNA或DNA,以進行雜合,以自動顯影技術觀察
- Blast analysis displayed one of the Northern blot positive clone is homologous with autotaxin gene, and another is homologous with calcyclin gene. 對這13個克隆進行測序和Blast同源序列分析表明,其中一個Northern雜交陽性克隆與autotaxin基因同源,另一個與鈣周期蛋白基因同源。
- Animals were killed at day 3,7,14and 28 after BLM administration. The total RNA was extracted from the lung tissue. The expressions of SPsmRNA were analyzed with Northern blot. 方法氣管內灌注博萊黴素A5,複製肺纖維化模型,分別於3、7、14和28天處死動物,提取肺組織總RNA,進行Northern雜交。
- Expression of messenger endothelin 1 (ET 1) RNAs and its receptor subunits (ETA,ETB)were studied by northern blot hybridization in rat kidney following renal ischemia reperfusion. 為了探究內皮素?1(ET?1)對腎功能的影響和作用方式,採用斑點雜交和原位雜交方法對大鼠缺血60分鐘再灌注腎組織ET?1及其受體亞型(ETA、ETB)的基因表達進行了研究。
- Kumark Saivithriy S. MRNA levels of Ca2+-independent form of protein kinase C in poslinchemic gerbil brain by northern blot analysis [J],J Biol Chem, 1997,252(61):7603. 盧布峰;魯友明;黃詒森;蛋白激酶C對大鼠腦缺血海馬突觸體谷氨酸攝取的調控作用[J].;生物化學雜誌;1994;10(3):371
- The supernatant of 20% brain tissue suspension of suckling mice infected with BA66019 strain of XHF virus, were separated by electrophoresis of SDS-PAGE and bloted in NC membranes by western blot technique . 用新疆出血熱病毒原型毒株BA66019感染乳鼠腦的20%25懸液,經差速離心后的上清為抗原進行SDS-PAGE電泳,再用Westernbolt法將凝膠分離的結果印跡到NC膜上。 將6株單克隆抗體小鼠腹水經飽和硫酸銨純化后,在NC膜上進行結合反應。
- Methods: In patients with AML M2b, karyotype was studied by using G banding technique, AML1/MTG8 fusion gene transcript by using RT PCR and the rearrangement of AML1 gene and MTG8 gene by using Southern Blot technique. 方法 :應用 G顯帶技術分析染色體核型 ,用 RT- PCR法檢測 AML 1/ MTG8融合基因轉錄本並進行微量殘留白血病檢測 ,採用 Southern Blot技術檢測 AML 1及 MTG8基因重排。
- Northern blot indicate that human BRI_3 mRNA is expressed principally in the brain, with the highest levels found at the cerebral cortex, medulla, amygdala, hippocampus, thalamus, caudate nucleus and spinal cord. BRI_2基因單鹼基突變造成的通讀和核苷酸重複,是基因水平上導致這兩種痴呆症的主要原因。 該基因屬於BRI基因家族的一員,目前得到分離鑒定的該家族成員包括BRI_1,BRI_2和BRI_3。
- Northern blot showed that A4-1 clone was specifically expressed in the antenna of H. armigera and the expressed level was higher in male antenna than that in female ones, which was similar to the expression of PBP-Harm gene from cotton bollworm. Northern雜交表明,克隆A4-1在棉鈴蟲觸角中專一性表達,並且在雄蛾觸角中的表達量明顯比雌蟲中高,這與棉鈴蟲性外激素結合蛋白基因的表達相似。
- Results of Northern blot showed that GOBP2 gene expressed specifically in the antenna of SPodoPtera exigua, and the expression level is consistent in the antenna of male and female moths. Northern雜交結果表明,GOBP2基因在甜菜夜蛾觸角中特異性表達,並且在雌雄蛾觸角中表達水平相當。
- We obtain 15 differentially expressed genes to human glioma thruogh four times hybridizations and scanning. Northern blot analysis confirmed 436F11 clone was over expression in human brian tissue and low expression in human glioma tissues. 通過四次基因晶元篩選 ,獲得 15條與膠質瘤相關的新基因 ,經northernblot證實 436F11基因在人正常腦組織中高表達 ,而在人腦膠質瘤中低表達。
- The expression of the novel gene in 12 specimens of pancreatic cancer and normal tumor-surrounding pancreas tissues and 4 pancreatic cancer cell lines(PaTu8988,SW1990,BxPC-3,AsPC-1)were detected by RT-PCR and Northern blot analysis. 應用RT-PCR和Northernblot檢測新基因在12例胰腺癌和癌旁正常胰腺組織以及4種胰腺癌細胞株(PaTu8988、SW1990、Bx-PC-3、AsPC-1)中的表達情況。