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- 人U6啟動子human U6 promoter
- U6啟動子U6 promoter
- 方法構建利用U6啟動子轉錄功能性小干擾RNA(siRNA)的質粒載體,對陽性克隆進行酶切和測序鑒定后,轉染人甲狀腺癌FRO細胞,利用RT-PCR和W eston blot法檢測hTERT基因的表達情況。Methods A vector containing U6 promoter used to transcribe functional small interfering RNA(siRNA) targeting hTRET gene was constructed. After identification by restriction enzyme digestion and sequencing,the plasmid of positive clones was transfected into human thyroid carcinoma FRO cell line. The expression of hTRET gene was detected by RT-PCR and Western blot.
- 子midnight
- 啟動to start
- 方法:設計和構建由U6啟動子驅動產生hTERT的siRNA表達質粒,轉染至ALVA-41前列腺腫瘤細胞,以TRAP-ELISA方法觀察細胞端粒酶活性,W estern-b lot檢測端粒酶蛋白的表達,細胞記數法檢測對細胞生長的影響。Methods:A plasmid including U6 promoter and siRNA of hTERT was designed and constructed. The plasmid was transfected into ALVA-41 cell line. The telomerase activity was tested by telomerase repeat amplification protocol ELISA(TRAP-ELISA).
- 重新啟動reboot
- 啟動子promotor
- 人黑素瘤細胞A375 MIA/CD-RAP基因啟動子的克隆及其鑒定Cloning of human MIA/CD-RAP promoter and its identification
- 人端粒酶逆轉錄酶基因啟動子區的克隆及其真核表達載體的構建Cloning of human telomerase reverse transcriptase gene promoter and construction of its eukaryotic expression vector
- 可及啟動子accessible promoter
- T7啟動子T7 RNA polymerase promoter
- TK啟動子TK promoter
- 基因外啟動子extragenic promoter
- 小啟動子minimal promoter
- A3啟動子actin 3 promoter
- C區啟動子core promoter
- KDR啟動子KDR promoter
- 35S啟動子35S promoter
- E8啟動子E8 promoter
