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- 花椰菜花叶病毒35S启动子cauliflower mosaic virus 35 S promoter
- 转基因食品的检测一般基于聚合酶链式反应(CR)法,对35S启动子和NOS终止子进行筛选。The method was based on using the polymerase chain reaction( PCR) to determine the35 S promoter and the NOS terminator for detection of GMOs.
- 结果显示,青枯病菌诱导的PPP1、PPP2和PPP3活性分别为35S启动子活性的53、39和25倍。HarpinXoo, DEG, DIR, or DPR activated PPP1 and PPP2 but not PPP3, consistent with the presence of Eli boxes in promoters.
- 以pC1303质粒为基础,构建了均由35S启动子驱动的CMO基因和BADH基因的植物双基因表达载体pC35SC35SB1303。A doublegene plant expression vector,pC35SC35SB1303 were reconstructed based on PC1303 plasmid by CMO and BADH which were both driven by 35S promoter.
- 我们利用此种芯片实现了对质粒pCAMBIA1301中所含的四个基因GUS、35S启动子、hpt、aadA的特异性同步检测。GUS,35S,hpt and aadA gene of plasmid pCAMBIAlSOl are simultaneously specifically detected by using arryed primer extension chip.
- 结论 改良CTAB法制备的DNA可用作PCR模板 ,建立的PCR检测camv 35S启动子和nos终止子方法可用于筛选食品中有无转基因成分S promoter,and 1%25 RRS could be indentified by PCR detecting nos te rminator. Conclusion DNA extracted from genetically modified food b y using modified CTAB methods can be used as template for PCR,the PCR method of d etecting camv 35? S promoter and nos terminator can be used for screenin g genetically modified foods.
- 启动子promotor
- 为了鉴定DFL基因的功能作用,构建了由35S启动DFL基因的正反义表达载体(载体中同时包含有由另外两个35S启动的潮霉素筛选基因和GUS组织化学染色基因)转化烟草。A full-length cDNA of DFL and its antisense nucleotide sequence have been transformed into Nicotiana tabacum under the control of a cauliflower mosaic virus 35S promoter.
- 可及启动子accessible promoter
- T7启动子T7 RNA polymerase promoter
- TK启动子TK promoter
- 基因外启动子extragenic promoter
- 小启动子minimal promoter
- A3启动子actin 3 promoter
- C区启动子core promoter
- KDR启动子KDR promoter
- 35S启动子35S promoter
- E8启动子E8 promoter
- U6启动子U6 promoter
- AFP启动子AFP promoter
