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- Construction, expression and identification of anti-human AFP VH single domain antibody fusion protein gene 抗AFP重链可变区单域抗体融合蛋白的构建、表达及初步鉴定
- Construction of Genetically Engineered Antibody Fusion Proteins 基因工程抗体融合蛋白的构建
- antibody fusion protein 抗体融合蛋白
- Purified fusion protein was obtained. 得到纯化的融合蛋白。
- Mouse polyclonal antibody against GST-Norpeg fusion protein was prepared with purified GST-Norpeg fusion protein as immunogen. 以纯化的重组目的蛋白作为免疫原免疫小鼠，制备相应的多克隆抗体。
- Othermore, plasmid phIgVhB7DC was transiently transfected into the 293T cells, and hB7DChIg fusion protein was obtained for monoclonal antibody preparation and screening. 随后，通过将质粒phIgVhB7DC瞬时转染293T细胞，收获hB7DChIg融合蛋白用于单克隆抗体的制备和筛选。
- The GRA7 gene may be expressed as a GST fusion protein in E. coli. GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达。
- Remarkable immunogenicity of the TrxA/PEB1 fusion protein was well identified: A high titer of the specific antibody to CJ Pen19 whole antigen weredemonstrated, which could cross-reacted with purified TrxA/PEB1 fusion protein. （3）证实TrxA/PEB1融合蛋白有高度特异的免疫原活性： CJ Pen19全菌抗原免疫后4周在雄性新西兰兔诱发高滴度特异性抗体，可同时与Pen19菌体粗提蛋白和TrxA/PEB1蛋白发生特异性结合。
- Methods Expression vector pCTLA 4/Ig was transfected into COS 7 with lipid reagent. The expression of fusion protein in the supernatant of the cell culture media was detected with double antibody sandwich ELISA. 方法 将pCTLA 4/Ig表达质粒转染COS 7细胞 ,双抗体夹心ELISA检测细胞培养上清中融合蛋白表达 ;
- Preliminary western blot result indicated that the polyclonal anti-GST/Ag85B antibody could recognize Ag85B/IL-II fusion protein which was prepared by our department,and showed high specificity. 酶联免疫测定法(ELISA)鉴定抗体,证明制备的多克隆抗体可以与 GST-Ag85B融合蛋白相结合,产生抗原-抗体反应。
- Conclusion:The purified fusion protein has good fineness and hemolysis activity, the antibody titers initiated by the protein vaccine go with regulation and the immunoprotection is satisfied. 结论:纯化的目的蛋白达到电泳级纯度，且依然保持良好的黏附活性，蛋白疫苗作用机体产生的抗体水平符合抗体消长规律。
- Western Blotting of the two kind of fusion protein further displayed specific bands at the anticipated position, attesting that the fusion protein could react with antibody specifically. 用His-tag抗体进行Western-Blot检测，分别在预期的位置发现特异的条带,说明融合蛋白能与抗体进行特异性结合。
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF亲和层析柱纯化融合蛋白;
- The fusion protein was purified through Nickel-affinity chromatography column. 用镍柱纯化融合蛋白。
- Methods The recombinant fusion protein MBP hEra was expressed in the E. 方法在大肠杆菌中,表达重组MBP?hEra融合蛋白。
- BVP22 can mediate intercellular trafficking of fusion protein in vitro and in vivo. 在体内外BVP22均能够介导与其融合的蛋白在细胞间发生穿梭。
- The titer of antiserum was detected by double agar immunodiffusion with GST-PI fusion protein. 并用双向免疫扩散法鉴定家兔多价抗血清的效价。
- EGF-Ang fusion protein had definitely cytotoxicity to Hep2 cells which over expressed EGFR in vitro. 融合蛋白EGF-Ang在体外对过度表达EGFR的Hep2细胞具有明显的杀伤作用。
- These fusion proteins were used to immunize rabbits to raise antisera. 这些表达产物用于免疫动物产生多克隆抗血清。
- Recombinant TrxA/PEB1 fusion protein could be a valuable candidate for CJ vaccine with great applied value. （4）PEB1融合蛋白作为CJ的亚单位侯选基因工程疫苗不仅可行，而且有良好应用前景。
