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- Results Human motilin receptor was successfully cloned with correct open reading fragment. 结果成功克隆了人胃动素受体基因表达片段,测序证实读框正确。
- Opening reading fragment (ORF) 开放性读码框
- Using genome blast, MAGE-H1/Apr-1 was mapped to chromosome Xp11.22 with the open reading frame (ORF) locating in one exon. 进一步在人基因组数据库利用Blast比较的方法将MAGE-H1/Apr-1定位在Xp11.;22。
- The recombinant palsmid(pGEX-4T-2/ IL-15) was obtained and sequence analysis verified its opening read fram(ORF) is correct. 经酶切和测序分析,表明成功构建了pGEX-4T-2/IL-15原核表达克隆;
- Here, we describe the screening of 10 patients who presented with 46, XY sex reversal for mutations in open reading frame (ORF) of SRY gene. 通过筛查10位中国46,XY女性性反转病人SRY基因的开放阅读框区域,探寻新的突变类型。
- According to the data of TMV MP in the GeneBank, design gene specific primer, amplified of the whole open reading frame (ORF). 根据GeneBank中TMV MP的序列设计基因特异引物,扩增出运动蛋白基因的整个开放阅读框(ORF)。
- An open reading frame (ORF) is a portion of a gene's sequence that contains a sequence of bases, uninterrupted by stop sequences, that could potentially encode a protein. 开放阅读框是基因序列的一部分,包含一段可以编码蛋白的碱基序列,不能被终止子打断。
- Each open reading frame (ORF) is knocked out using a PCR-based gene deletion strategy that takes advantage of the high degree of homologous recombination in yeast. 每个开放读码框使用基于PCR基因删除策略,使用在酵母中的高度同源重组优势。
- An open reading frame (ORF) is a portion of a gene's sequence that contai a sequence of bases, uninterrupted by stop sequences, that could potentially encode a protein. 开放阅读框是基因序列的一部分,包含一段可以编码蛋白的碱基序列,不能被终止子打断。
- A synthetic peptide derived from the C terminal sequence of the open reading frame(ORF)j5R was recognized by antisera from pigs recovered from infection with different African swine fever virus(ASFV)strains. 根据非洲猪瘟病毒MalawiLIL20/1株j5R阅读框C端抗原决定簇序列制备的合成肽能被不同毒株感染康复猪的免疫血清识别。
- Based on the position weight matrix(PWM) and length distribution of open reading frame(ORF),a simple method for predicting translation initiation sites is presented. It can identify TIS from upstream AUGs easily. 结合位置权重矩阵(PWM;position weight matrix)和开放阅读框架(ORF;open reading frame)的长度分布特征建立了简单的方法识别翻译起始位点;此方法能很好地区分上游AUG和TIS.
- The full-length cDNA of the OsBIRF1 is 1837 bp with a predicted 1191bp open reading frame (ORF), which predicts to encode a 396 amino acid protein containing all conserved domains of the RING finger protein. OsBIRF1基因全长cDNA为1873bp,包含一个1191bp的ORF,编码一个含396个氨基酸的蛋白,并含有RING指蛋白所有的特征性保守结构域。
- Using the Index of Coincidence to identify Open Reading Frames. 使用偶合指数方法来定出开放阅读框架。
- The growth hormone cDNA gene (cgh) of grassfish (Ctenopharyngodon idellus) was obtained by RT-PCR, the sequence analysis showed that the ORF (open reading frame) of cgh gene, encoding 210 amino acid residences, was 633 nucleotides. 通过RT-PCR获得草鱼(Ctenopharyngodon idellus)生长激素cDNA基因(cgh);序列研究表明;cgh基因的ORF为633nt;编码210aa.
- AmphiANT, was identified from the gut cDNA library of amphioxus B. becheri tisingtauense, consists of 1289 bps and contains an 897 bps open reading frame (ORF) corresponding to a deduced protein of 298 amino acids. AmphiANT全长1289 bps,含有一个897bps的开放阅读框,编码一个298个氨基酸组成的蛋白质。
- The SJCWL06 gene is 861 bp in whole length, has an open reading frame (ORF) of 327 bp encoding 109 amino acids with a signal peptide, an ABC-ATPase domain, several phosphorylation sites and the highest similarity of SMC3 protein of Apis mellifera (84%). 该基因长861bp,有一个完整的327bp的开放读框,编码109个氨基酸,其编码蛋白有一个信号肽,多个磷酸化位点和一个ABC-三磷酸腺苷酶的结构域,与蜜蜂(Apis mel-lifera)SMC3编码的mRNA基因序列同源性最高(84%25)。
- The ZFP28 cDNA sequence is 4104bp and contains a 2076-bp open reading frame (ORF) that encodes an 868 amino acid protein with an N-terminal signal peptide, two KRAB domains and 14 C-terminal C2H2 zinc finger motifs. ZFP28长4104个碱基,含有一个长为2076个碱基的开放阅读框,编码一个长为868个氨基酸的蛋白质,它包含一个N-端信号肽,两个KRAB结构域和14个C-端C2H2型锌指结构。
- The results were summarized as follows: A sequence encoding mannanase, which containing a 1233 bp open reading frame (ORF), was predicted according to the oligonucleotides homologous from LeMan1. 结果如下: 参考番茄中编码甘露聚糖酶的基因序列,通过电子克隆的方法预测出一条编码大豆甘露聚糖酶的核苷酸序列,设计特异引物,采用RT-PCR方法扩增出一条1233 bp的cDNA,命名为GmMan1。
- A full length of NCED cDNA, namely SgNCED1, was cloned and sequenced.It was 2241-bp long and contained an open reading frame (ORF) of 1809-bp which encodes a peptide of 602 amino acids. 1、 柱花草SgNCED1基因的克隆 根据已报道的三种豆科植物的NCED基因同源序列设计简并引物,用RT-PCR结合RACE以及嵌套PCR的方法,从水分胁迫的柱花草叶片中克隆了SgNCED1基因,其cDNA全长由2241 bp组成,其开放阅读框架为1809 bp,编码602个氨基酸。
- CHS gene was cloned from cDNA and CHS open reading frame sequence was obtained. 获得了CHS基因ORF(Open reading frame)的cDNA序列,全长为1299bp。