The results were summarized as follows: A sequence encoding mannanase, which containing a 1233 bp open reading frame (ORF), was predicted according to the oligonucleotides homologous from LeMan1.

• 结果如下： 参考番茄中编码甘露聚糖酶的基因序列，通过电子克隆的方法预测出一条编码大豆甘露聚糖酶的核苷酸序列，设计特异引物，采用RT-PCR方法扩增出一条1233 bp的cDNA，命名为GmMan1。