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- Keywords NDRG1;GST;gene expression;glutathione sepharose 4B; 基因表达;谷胱甘肽琼脂糖-4B;
- Glutathione Sepharose 4B 谷胱甘肽琼脂糖凝胶
- Made sheep anti?human IgG Fab Sepharose 4B affinity chromatography, purified the souble Fab fragment. 制备羊抗人IgGFab抗血清、建立抗人IgGFab抗体－Sepharose4B亲和层析柱，纯化Fab片段。
- The coupling ratio between monoclonal antibodies and Sepharose 4B is 85.21%. The active groups on the Sepharose 4B base materials are blocked and washed,stored. 取经溴化氰活化的Sepharose 4B与单克隆抗体抗体偶联;偶联比为85.;21%25;封闭活化位点;洗涤;制备IAC柱。
- A trypsin like enzyme(AGP, Mr 14kD) was purified after ammonium sulfate precipitation, Sephadex G 75 gel filtration, Sephadex DEAE A 25 ion exchange chromatography and SBBI Sepharose 4B affinity chromatography. 肠液经硫铵沉淀，SephadexG?75凝胶过滤，SephadexDEAEA?25离子交换和SBBI?Sepharose4B亲和层析，分离纯化出分子量约为14kD的巨尾阿丽蝇蛋白酶。
- SDS PAGE and Dot blot indicated that this 12kD protein was PF4.ConclusionUsing SZ 95 sepharose 4B affinity chromatography to purify PF4 is an efficient method to prepare PF4 with high biological activity. 结论用SZ 95 Sepharose 4B亲和色谱柱纯化的PF4产品得率高、纯度高、活性好
- Methods: EPF was purified from 430ml human pregnancy sera. The purification scheme involved sequential DEAE 52 chromatography, SP Sepharose F. F chromatography, ConA Sepharose 4B chromatography and Heparin Sepharose C1-6B chromatography. 方法:从430ml早孕血清中采用柱层析方法分离纯化EPF,依次经过DEAE 52阴离子交换层析、SP Sepharose EF阳离子交换层析、ConA Sepharose 4B亲和层析和Heparin Sepharose C1-6B亲和层析。
- Methods:Triton/tris was used to extract the crude H 2,to which of the anti H 2 antibodies coupled with sepharose 4B was applied to purify H 2 antigen by affinity chromatography. The coupled H 2 Ag was eluted via 0.5%DOC,0.65M NaCl solution. 用0.;5%25Triton/Tris抽提小鼠组织相容性抗原（H－2）抗原，利用抗H－2抗原抗体制备的亲和柱，特异性结合H－2抗原，再用0
- MethodsMonoclonal antibody is an IgG1 antibody, sepharose 4B and SPA affinity column were applied for purifi cation of AchE monoclonal antibody (3F3), using papain cut 3F3,to get Fab fragme nt and immune mice with Fab. 方法 AchE单克隆抗体是一种IgG1抗体 ,先用木瓜蛋白酶酶切 ,然后采用AchE Sepharose 4B和SPA亲和层析柱进行提取纯化 ,获得纯化的单克隆抗体Fab段。
- Then hemalbumin was immobilized to the activated vehicle Sepharose 4B with the couple ratio of 5.3mg hemalbumin per gram vehicle to make hemalbumin column, which was applied to measure trace hydrogen peroxide. 将血红白蛋白固定在已活化的Sepharose 4B载体上制成血红白蛋白柱子，偶联率达每克载体结合5.;3mg血红白蛋白。
- Sepharose 4B column attached with spacer arms used in affinity chromatography of anti hCG antibody 用接手臂的Sepharose 4B柱亲和层析纯化抗人绒毛膜促性腺激素抗体
- Purification of a Phospholipase A_2 from Bungarus fasciatus Venom by One Step Sepharose 4B Method Sepharose 4B一步法对金环蛇蛇毒磷脂酶A_2的分离纯化
- sepharose 4B 琼脂糖珠, 琼脂糖(凝胶)4B
- After purification with DEAE Sepharose F. 结果：成功得到海螵蛸粗多糖。
- China recovers $4b embezzled in 2007. 中国收回相当于40亿美元在2007年被盗用的资金。
- Glutathione peroxidase( GSH-Px) acts as a free radical scavenger. 谷胱甘肽过氧化物酶是生物机体内的自由基清除剂。
- At the same time, glutathione homeostasis is disturbed (6-9). 在此同时（研究6~9）发现谷胱甘肽的稳定状态受到了干扰。
- The liver is particularly rich in glutathione for this purpose. 因为这个原因肝脏的谷胱甘肽含量特别高。
- Glutathione is also the main detoxifying agent in the body. 谷胱甘肽也是身体主要的解毒剂。
- The reaction involves oxidation of endogenous glutathione to its disulfide. 该反应是内源性谷胱甘肽氧化成了它的二硫化物。
