Methods:Triton/tris was used to extract the crude H 2,to which of the anti H 2 antibodies coupled with sepharose 4B was applied to purify H 2 antigen by affinity chromatography. The coupled H 2 Ag was eluted via 0.5%DOC,0.65M NaCl solution.
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- 用0.;5%25Triton/Tris抽提小鼠组织相容性抗原(H-2)抗原,利用抗H-2抗原抗体制备的亲和柱,特异性结合H-2抗原,再用0