Then hemalbumin was immobilized to the activated vehicle Sepharose 4B with the couple ratio of 5.3mg hemalbumin per gram vehicle to make hemalbumin column, which was applied to measure trace hydrogen peroxide.

• 将血红白蛋白固定在已活化的Sepharose 4B载体上制成血红白蛋白柱子，偶联率达每克载体结合5.；3mg血红白蛋白。