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- The most widely used animal serum is fetal bovine serum, FBS. 目前应用最广泛的动物血清是胎牛血清。
- The fetal bovine serum( FBS) has been widely used in biomedical research. 胎牛血清在生物医学研究中广泛应用。
- SMC rapid proliferation was brought by stimulation of 20% fetal bovine serum (FBS). 用 2 0%25胎牛血清刺激SMC快速增殖。
- Methods Human gastric cancer cell AGS were cultured in a RPMI 1640 medium with 10% fetal bovine serum(FBS). 方法胃癌细胞株AGS于含10%25胎牛血清的RPMI1640培养液中常规培养,AGS细胞培养24h至对数生长期改用无血清RPMI1640培养基培养24h加入干预药物。
- Chondrocytes grew well in F12 medium supplemented with 10% fetal bovine serum (FBS). 软骨细胞在加有10%25胎牛血清的F12培养液中生长良好。
- DMEM with 2% fetal bovine serum was used to induce MDSCs to differentiate into skeletal muscle lineage. 用含2%25胎牛血清的DMEM培养基促进其向骨骼肌细胞分化。
- A subpopulation of cells differentiated into adipocyte-like cells when cultured in 10% fetal bovine serum. 在含10%25胎牛血清的培养基中培养时，细胞可以分化成类脂肪细胞。
- Meterial and Methods:Using different medium and concentriation of fetal bovine serum,we have modified the method to culture the primary HEL,HEK. 方法：分别用不同的培养基及不同浓度的血清，以改进的方法对原代人胚肺、肾细胞进行培养。
- METHODS: AGS cells were cultured in RPMI-1640 medium with 100 mL/L fetal bovine serum (FBS), 100 kU/L penicillin and 100 kU/L streptomycin. 方法:AGS在含100 mL/L小牛血清+100 kU/L青、链霉素的RPMI1640培养基中常规培养.
- Results:The lung and kidney from 3 months embryo fetal were culture with MEM or RPMI 1640 and 20%fetal bovine serum,cellular adhersion and growth were well. 结果：经水囊引产的3月大小的胚胎肺及肾，用MEM及1640加上20%25的牛血清，对细胞的贴附及增殖十分有利，细胞生长状态良好。
- Method: The effect of different cell density, different concentrations of fetal bovine serum (FBS), EGF and PDGF-BB on hMSCs proliferation was assessed by MTT assay. 方法：采用MTT比色法检测不同接种密度、不同浓度的胎牛血清、EGF和PDGF-BB对MSC生长的影响；
- OBJECTIVE To observe the effects of fetal bovine serum (FBS) and artificial perilymph (APL) on the differentiation of neural stem cells from newborn fetal guinea pigs. 目的体外定向诱导新生豚鼠海马神经干细胞分化为内耳毛细胞。
- Conclusions:This results showed that medium of MEM and 1640 containing 20%fetal bovine serum were benifit for the culture of primary embryo fibroblast cell and kidney cell. 结论：经改进后的对人胚肺细胞和人胚肾细胞的原代培养，用含20%25的小牛血清的MEM和1640的培养液对细胞的生长极为有利。
- The effects of fetal bovine serum(FBS) addition time,concentration and different culture systems on the developmental potentiality of bovine parthenogenetic embryos were observed. 试验研究了培养液中血清的添加时间和添加浓度及不同培养体系对黄牛孤雌胚胎体外发育潜力的影响。
- The cell number of blastocysts obtained from in vito/in vitro were counted. Results The ratio of blastocyst formation was improved in KSOM and CZB by addition of fetal bovine serum(14.71% vs 85.71%,6.45% vs 10.81%). 结果 在KSOM和CZB中添加胎牛血清能显著提高胚胎囊胚发育率 (14 71%25对 85 71%25 ;6 4 5%25对10 81%25 ) ;
- The study was to examine the effect of panaxynol on fetal bovine serum (FBS)-induced proliferation of primary cultured Sprague-Dawley rat aortic smooth muscle cells (RASMCs) and the possible pathway. 本实验旨在明确panaxynol对血清诱导大鼠胸主动脉平滑肌细胞(rat aortic smooth muscle cell, RASMCs)增殖的影响及可能的途径。
- Methods The Hepatitis B virus transfected hepatoblastoma cell line HepG2.2.15 which can consistently produce Dane particles was maintained in DMEM medium containing 380g/ml G418 and 10% fetal bovine serum . 方法 以HepG2.;2
- Methods The growth effects of EGF and NC on U251 MG cultured in serum-free and serum containing (10% fetal bovine serum, FBS) media respectively were observed by MTT colorimeritric analysis. 方法 :在含 10%25小牛血清和无血清培养基中 ,加入不同浓度的EGF ,NC及NC和EGF(10ng·ml-1)干预 ,应用MTT法观察其细胞生长效应。
- heat-inactivated fetal bovine serum 热灭活胎牛血清
- veronal-buffered saline:fetal bovine serum(barbital) 维罗那缓冲盐水与胎牛血清(巴比妥)之比
