Methods The growth effects of EGF and NC on U251 MG cultured in serum-free and serum containing (10% fetal bovine serum, FBS) media respectively were observed by MTT colorimeritric analysis.

• 方法 :在含 10%25小牛血清和无血清培养基中 ,加入不同浓度的EGF ,NC及NC和EGF(10ng·ml-1)干预 ,应用MTT法观察其细胞生长效应。