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- DNA测序的通用引物universal primer
- (DNA测序的)通用引物universal primer
- 利用ITS的通用引物(ITS5-ITS4)对云南的美味牛肝菌(Boletusedulis)子实体的DNA进行PCR扩增,扩增产物回收后直接测序。A pair of general primer (ITS5-ITS4) was used to test in amplification of internal transcribed spacer (ITS) region of chromosomal DNA of fruiting bodies of Boletus edulis in Yunnan.
- DNA测序引物sequencing primer
- 在SR1基因两端设计引物,对绥农10号基因组进行了扩增,经测序获得3972bp的DNA序列SR2。The gene has been submitted to the GenBank database, and the accession number is AY193892.3 Designed specific primers according to the sequence of SR1, a PCR reaction was done with the genome DNA of Suinong10 as template.
- 应用ABI PRISM~(TM)310测序仪进行快速且经济的DNA测序Rapid and Economic DNA Sequencing Using ABI PRISM~(TM) 310 Sequencer
- 开发Unicode是为了创建能容纳多数已知脚本的通用字符集。Unicode was developed to create a universal character set that can accommodate most known scripts.
- 通用引物universal primer
- 综合介绍DNA测序中常用毛细管凝胶电泳 (CGE)分离技术。The CGE separation technique usually used in DNA Sequence Determination is introduced in detail in this paper.
- 真菌特异性通用引物的聚合酶链反应系统的实验与临床研究Experimental and Clinical Study on Detection of Medically Import ant Fungi by PCR with A Universal Fungus-specific Primer System
- DNA测序、筛选cDNA文库的加减法plus-minus method
- 啤酒污染乳酸菌PCR引物的设计The PCR Primers Design Of Beer Spoilage Lactic Acid Bacteria
- 方法:用血清学血型方法、PCR-SSP法和ABO基因第6及第7外显子直接测序的方法对B(A)血型和B(A)型等位基因进行检测。Methods:ABO blood groups were identified by serological tests. B(A) alleles were determined by PCR-SSP and direct DNA sequencing at exons 6 and 7 of ABO gene.
- 方法用1、2、3类三种整合酶基因通用引物扩增184株中段尿中分离革兰阴性杆菌的相应基因;Methods To amplify the class1,2 and 3 integrase genes in 184 gram negative bacilli from medistream urine by polymerase chain reaction with universal primer of 3 genes.
- 成功扩增出HBeBP4A基因,测序结果符合GenBank报告序列。HBeBP4A gene was successfully amplified and identified by DNA sequencing.
- 例如,在过去的两年中人们已经借助于MGR和GRIMM软件得到了大鼠与人以及最近新测序的挪威鼠之间的几个比较有趣的进化方案。For example, in the last two years, several very interesting evolution scenarios have been proposed between the mouse and the human, and between the mouse and the newly sequenced Norway rat, using the MGR and GRIMM software.
- 皱纹盘鲍(Haliotis discus hannai)微卫星DNA的筛选与引物设计Isolation and Primer Designing of Microsatellite Marker in the Pacifi Abalone(Haliotis discus hannai)
- 方法根据GenBank S.suis2epf基因序列设计引物,克隆ZYH24株epf基因片段并进行序列分析;Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf.
- 方法利用酶切及SSCP技术检测凝固酶基因序列改变情况,并测序进行确证。Methods DNA sequences of the coagulase were examined by polymerase chain reaction-sigle strand conformation polymorphism (PCR-SSCP) method and were identified by sequencing.
- 方法:应用RT-PCR结合测序方法研究白血病细胞系K562和CEM/VLB100APAF-1cDNA类型;Methods:APAF-1 cDNA types in leukemic cell lines K562 and CEM/VLB100 were studied by cDNA cloning strategy.