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- DNA测序引物sequencing primer
- 参考GeneBank中ARV-S1133株S1基因序列设计一对测序引物,一对诊断引物。According to the published S1 gene sequence of ARV-S1133 strain in GeneBank,two pairs of primer were designed,one is sequencing primer,the other is diagnostic primer.
- DNA测序的通用引物universal primer
- 本实验将我国FPV282E4 株基因组3 .6kb BamHID片段利用DNA测序仪进行了序列测定。The nucleotide sequence of BamHI genomic fragment from fowlpox virus(FPV)strain 282E 4 has been determined by ABI PRISM TM 377 DNA sequencer.
- 引物primer
- DNA测序正确后,将IL-29 cDNA的编码框序列构建到真核表达载体psectagB/His-myc中。The recombinant expressing plasimd of psectagB/His-myc-IL29 was constructed by inserting IL-29 cDNA into the vector and was then transfected into cos-7 cells.
- DNA测序法DNA sequencing
- 应用ABI PRISM~(TM)310测序仪进行快速且经济的DNA测序Rapid and Economic DNA Sequencing Using ABI PRISM~(TM) 310 Sequencer
- 反向测序引物reverse sequencing primer
- 综合介绍DNA测序中常用毛细管凝胶电泳 (CGE)分离技术。The CGE separation technique usually used in DNA Sequence Determination is introduced in detail in this paper.
- 测序引物sequencing primer
- DNA测序分型DNA sequencing based typing (SBT)
- DNA测序梯sequencing ladder
- DNA测序仪DNA sequencer
- 根据测序结果设计新的引物以进行热启动PCR反应;New primers were designed for hot - start PCR reaction according to the results of DNA sequencing.
- 桑格DNA测序法Sanger method
- 链终止(DNA测序)法chain termination method(Sanger method); Sanger method
- 方法用一对引物进行PCR扩增,扩增产物纯化后用双脱氧终止法测序。The PCR products were purified and sequenced by the methods of Sanger dideoxy.
- 化学降解(DNA测序)法chemical degradation method(Maxam-Gilbert method); Maxam-Gilbert method
- 双脱氧链终止DNA测序法dideoxy chain-termination method(Sanger method)