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- Keywords Avian influenza;H5 subtype;Codon optimized HA gene;DNA vaccine;Immunoprotection; 禽流感;H5亚型;密码子优化的HA基因;DNA疫苗;免疫保护;
- Codon optimized HA gene 密码子优化的HA基因
- H7N1 AIV HA gene was synthesized from A/African starling/983/79(H7N7) cDNA template. 以A/African Starling/983/79(H7N1)毒株为模板，人工合成了H7亚型AIV的HA全基因序列，并将其克隆于pMD18-T载体。
- We used the same method as above-mentioned and obtained the recombinant fowlpox virus expressing antigen part of HA gene, rFPV-12LS-HAA. 将质粒pcR2.;1一HAA用BamHI酶切;回收纯化1
- This article reviews the codon bias and some strategies for codon optimization. 本文就密码子的偏好性及密码子优化的一些策略简要的进行了综述。
- One involves the HA gene: to become active in a cell, the HA protein must be cleaved into two pieces by a gut-specific protein-cutting enzyme, or protease, supplied by the host. HA蛋白要想在细胞内发挥作用，必须由宿主肠道才有的某个蛋白分解酵素切成两段。
- In this study, the influenza virus A/Equine/Jingfang/74-1(HTNT)was propagated in SPF egg embryo, and RNA was extracted with trizol LS reagent, then HA gene was amplified, cloned and sequenced. 本研究应用无特定病原体(SPF)鸡胚增殖马流感病毒A/马/京防/74-1(H7N7)毒株,TRIzol LS Reagent提取病毒RNA,RT-PCR扩增HA基因全片段,克隆到PMD18-T载体上,并进行了鉴定和序列测定。
- Then the HA gene was sub-cloned into the vector pVAX1 at the downstream of CMV directedly. The recombinant, named pV-HA, was used as TIV DNA vaccine. F81 cells transfected with pV-HA did encode TIV HA protein specifically reacting to anti-TIV sera. 克隆了TIV HA基因并将其定向插入到真核表达载体pVAX中,得到基因疫苗表达载体pV-HA,将pV-HA 转染F81 细胞后,间接ELISA 试验结果表明,其能在F81 细胞中表达具有生物活性的HA蛋白。
- One pair of primers were designed on the basis of HA gene of AIV, allowing simultaneous detection of AIV subtype H9 and H5, and the specific amplicons were 579bp and 177bp in size respectively. 参照AIV的HA基因序列设计1对引物，对H9和H5亚型AIV进行了扩增，产物大小分别为579bp和177bp。
- For example, the use of speciesspecific codon optimization results in increased protein production on a per-cell basis, leading to enhanced T-cell responses and antibody induction. 例如，使用物种特异性密码子将会使每细胞单位的蛋白质产量增加，从而加强T细胞的反应和抗体的吸附。
- Ha has been seeing someone to the station. 他刚才送人上火车站去了。
- The boy is very active, ha never flays up. 这个男孩很活跃，但从来不惹麻烦。
- Codon Optimization of Antibacterial Peptide Magainin and CecA-mil Gene and Over -expression in Pichia pastoris Magainin和cecA-mil抗菌肽基因的密码子优化及在毕赤酵母中的高效表达
- Characterization of HA gene of caged pet birds H3N8 influenza A virus 宠物鸟H3N8亚型流感病毒HA基因的特征性分析
- We optimized the codon usage of wild type HPV 16 L1 gene according to the codon bias of mammalian,and synthesized the full-length optimized L1 gene,named mod. 首先按照哺乳动物密码子偏好对野生型HPV16 L1基因进行改造并合成优化基因;命名为mod.;HPV16L1。
- codon optimized 密码子优化
- We hummed and ha'd for ages before deciding to buy the house. 我们犹豫了很久，才决定买这所房子。
- Codon Optimization and Expression of Norovirus Capsid Proteins in Insect Cells 人诺如病毒衣壳蛋白的密码子优化及在昆虫细胞中的表达
- A triplet of nucleotides; a codon. 三核苷酸，密码子
- Max Zorin: Ha ha, you amuse me, Mr. Bond. 佐林：哈哈，你逗我，邦德先生。
