One pair of primers were designed on the basis of HA gene of AIV, allowing simultaneous detection of AIV subtype H9 and H5, and the specific amplicons were 579bp and 177bp in size respectively.

• 参照AIV的HA基因序列设计1对引物，对H9和H5亚型AIV进行了扩增，产物大小分别为579bp和177bp。