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- 长距离反向PCRlong distance-inverse PCR
- 巢式反向PCRnested inverse PCR
- 长距离反向散射long-distance-back scatter
- 采用反向PCR技术获得了该基因的编码区克隆。 分别构建含有内含子和不含内含子的AVA基因植物表达载体pBI121AVA-GUS和pBI121AVAc-GUS,通过根癌农杆菌介导法转化烟草。The AVA gene with and without intron were inserted downstream of 35S promoter into a vetor pBI121 to get pBI121AVA-GUS and pBI121AVAc-GUS vetor respectively.
- 方法在已成功克隆和表达人HMGB1的基础上,采用一步反向PCR致突变法构建人HMGB1cDNA的6个突变体,并分别克隆于原核表达载体pQE-80L上,表达6个相应的重组HMGB1突变体蛋白,并经Western blot鉴定。Methods This experiment was based on successful clone and expression of human HMGB1.Six mutant HMGB1 cDNA were designed and constructed by one step inverse PCR. They were cloned into prokaryotic expressive vector pQE80L and followed with production of mutant HMGB1s and identification by Western blotting.
- PCRpolymerase chain reaction (PCR)
- 反向辐射状免疫扩散reverse radial immunodiffusion ( RRID)
- PCR法PCR
- 作用于磁化材料以去除剩磁的反向磁通强度。The opposing magnetic intensity that must be applied to a magnetized material to remove the residual magnetism.
- 3-PCR3-PCR
- 反向递减reverse regression
- TD-PCRTD-PCR
- 棉条反向sliver reversal
- UP-PCRUP-PCR
- 反向旋转减少了净摩擦力。The counterrotation reduces the net friction torque.
- 组合PCRassembly PCR
- 集电结是反向编置。The collector junction biased in the reverse direction.
- 搭桥PCRbridge-PCR
- 反向带宽inverse bandwidth
- 荧光PCRfluorescent PCR