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- 巢式反向PCRnested inverse PCR
- PCR-流过式反向斑点杂交PCR-flow through reverse dot blot
- 压紧式反向接头imapct reverse sub
- 长距离反向PCRlong distance-inverse PCR
- 巢式PCRNested PCR
- 嵌入式flush bonding
- 巢式nesting
- 采用反向PCR技术获得了该基因的编码区克隆。 分别构建含有内含子和不含内含子的AVA基因植物表达载体pBI121AVA-GUS和pBI121AVAc-GUS,通过根癌农杆菌介导法转化烟草。The AVA gene with and without intron were inserted downstream of 35S promoter into a vetor pBI121 to get pBI121AVA-GUS and pBI121AVAc-GUS vetor respectively.
- 式的fashioned
- 巢式窖[化] beehive kiln
- 美式American
- 式中wherein
- 方法在已成功克隆和表达人HMGB1的基础上,采用一步反向PCR致突变法构建人HMGB1cDNA的6个突变体,并分别克隆于原核表达载体pQE-80L上,表达6个相应的重组HMGB1突变体蛋白,并经Western blot鉴定。Methods This experiment was based on successful clone and expression of human HMGB1.Six mutant HMGB1 cDNA were designed and constructed by one step inverse PCR. They were cloned into prokaryotic expressive vector pQE80L and followed with production of mutant HMGB1s and identification by Western blotting.
- 美式英语American English
- 嵌入式系统embedded system
- 巢床PCRnested PCR
- 日式Japanese
- 单式simplex
- 巴洛克式barpque
- 菜式cuisine
