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- 重叠延伸技术(SOE)splicing by overlap extension (SOE)
- PCR重叠延伸技术splicing by overlap extension
- 方法扩增EGFP及TK并采用重叠延伸拼接(SOE)法连接EGFP和TK,将EGFP-TK融合基因亚克隆至pBABE-puro-lox的Sal I和Cla I位点之间,构建成新载体pBGTKlox。METHODS:EGFP and TK were amplified by polymerase chain reaction and fused by splicing overlap extension. The fusion gene EGFP-TK was then subcloned to the sites Sal I and Cla I of pBABE-puro-lox to construct pBTKlox.
- 延伸技术Elongation technique
- 扩增前的引物延伸技术primer extension preamplification; PEP
- 重叠延伸PCRoverlap extension PCR
- 重叠延伸PCR法overlap extension by polymerase chain reaction
- SOE法SOE
- 重叠overlapping
- 几种分散控制系统的SOE性能测试比较Contrast on the Result of SOE Performance Tests of Several DCS
- DCS系统SOE性能的测试及分析Test and analysis of SOE performance in DCS
- 重叠延伸overlap extension
- 同时对光纤差动保护运用于线路变压器组时涉及的矢量变换、励磁涌流、TA饱和等延伸技术进行了分析,解决了传统的线路光纤差动保护不能满足线路变压器组保护的要求。The extending techniques such as vector transform, excitation inrush current , TA saturation and so on are analyzed, which resolves the question that the traditional optical differential protection can not satisfy the request of line-transformer unit.
- 重叠延伸拼接法splicing overlap extension; SOE
- SOE事件记录:在线记录事件量达64条,先进先出(IFO)态刷新,带有时间标记,掉电不丢失。SOE event record: can record up to64 online events, FIFO dynamic refresh, possess time mark, no lose when power supply drop.
- 我们缺少技术工人。We are short of skilled labor.
- 群山向西延伸。The mountains have a western trend.
- 重叠的overlapped
- SOE事件记录:在线记录事件量达64条,先进先出(FIFO)动态刷新,带有时间标记,掉电不丢失。SOE event record: can record up to 64 online events, FIFO dynamic refresh, possess time mark, no lose when power supply drop.
- 六年的待业并未使他的技术荒疏。A six-year lay-off has not rusted his skills.