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- 蛋白酶K法proteinase-K pathway
- 针对粘菌标本(孢子)数量少、形态微小等特点,分别采用CTAB法、SDS法、蛋白酶K法4和SDS-蛋白酶K4种方法,提取基因组DNA。Considering the fact that the specimens of myxomycetes are small in amount and minute in form, four methods, CTAB method, SDS method, protease K method and SDS - protease K method, are used to extract genomic DNA.
- 法law
- 无法unable
- 组织蛋白酶KCathepsin K
- 摘要梯度K法以欧氏距离公式为基础，应用于电测深找水，它能够利用实测数据较准确地确定最佳井位和估计涌水量，并获得较佳的地质效果。Based on Euclidean distance, applied in water detection by electrical sounding, The gradient K method can estimate the location of the well and its flowing yield with measured data, and can obtain satisfactory geologic results.
- 蛋白酶KProteinase K
- 说法statement
- 热硼酸／蛋白酶K法hot borate/Protease K method
- 输入法input method
- 除法division
- 经蛋白酶K消化处理的ECPs,银染可见脂多糖(LPSs)的典型O 糖侧链结构,显示脂多糖是组成粗制ECPs的重要组分之一。When treated with protoase K digestion and stained by silver assay, typical O-specific side chains of liposaccharides could be observed on the electrophoritic gel, which suggested that LPS was one of the most abundant component in the gross ECPs of Aeromonas hydrophlia.
- 分析法analytical method
- 商法commercial law
- 通过优化实验,确定了用SAP、Exo I和蛋白酶K联合使用对PCR产物进行纯化,可短时间、低成本、高效的获得PCR纯化产物;The method combines the SAP, Exo I and proteinase K to purify the PCR product, determined by our optimal experiment, can obtain the purified product with the short time, low cost and high efficiency;
- 宾夕法尼亚Pennsylvania
- 读法pronunciation
- 法轮功Falun Gong Cult
- 通过蛋白酶K消化过程中对于酶的浓度、温浴时间的比较试验,发现随着采用大幅提高酶浓度、延长消化时间等高强度的蛋白酶消化操作后,DNA的质量和产量均得到显著提高。We used three pretreatment methods to remove formalin:gradual dehydration by alcohol,GTE buffer displacement and critical point drying,by comparison the DNA from samples treated by critical point drying is the best in quality and quantity. Intensive proteinase K treatment including increased proteinase K concentration and prolonged digestion time also greatly enhances quality and quantity of DNA.
- 法门method
