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- 最小CMV启动子minimal cytomegalovirus immediate-early promoter
- 最小启动子minimal promoter
- 最most
- 子midnight
- 启动to start
- 最小化minimum
- 最小least
- 方法将含SFV复制子的元件从pSFV-MCS中切下,连入含CMV启动子及增强子的pIRESneo载体中,构建新型真核表达载体pCS,再将含HIV-1 MEGp24基因插入至pCS载体中,构建重组核酸疫苗pCS-MEGp24。Methods Digest plasmid pSFV-MCS with restriction endonuclease,and insert the obtained SFV replicon into vector pIRESneo containing CMV promoter and enhancer to construct a novel eukaryotic expression vector pCS. Insert HIV-1 MEGp24 gene into vector pCS,then transfect the constructed recombinant DNA vaccine pCS-MEGp24 to BHK-21 cells in mediation of liposome.
- 最小的lowest
- 最大的maximal
- 重新启动reboot
- 最大largest
- 最好had better
- 最多maximum
- 最好的best of all
- 启动子promotor
- 最小二乘法least squares technique
- CBA启动子引导的GFP表达效率高于CMV启动子。Expression efficiency of GFP driven by CBA promotor is highter than by CMV promotor.
- 最小公倍数lowest common multiple (L.C.M.)
- 小启动子minimal promoter