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- Growing transfected cells were selected by G418. 用G418筛选，建立转染克隆细胞系。
- The transfected cells expressed IL-18 fusion gene and 18 kD IL-18 protein. IL-18转染细胞表达IL-18融合基因及18 kD蛋白。
- To assay the transfected cells by immunohistochemical and insulin levels were monitored by radioimmunoassay( RIA). 并用放射免疫分析法检测细胞培养液胰岛素水平。
- FACS showed the C2 protein expression ratio of transfected cells was 32.3%, while the empty control group was 0.9%. 流式细胞分析法检测转染细胞C2蛋白表达率为32.;3%25，空白对照为0
- The transfected cells were inoculated into nude mice,and the tumor growth inhibition rate was calculated. 体外转染乳腺癌细胞,常规动物接种,测量肿瘤体积和动物重量,计算肿瘤抑制率。
- The pEGFPTTV2 was transfected into Cos7 cell line using LipoFectamine 2000, and the transcription products of TTV ORF2 gene in the transfected cells were confirmed by RT-PCR. 用脂质体介导法将pEGFPTTV2质粒DNA转染Cos7细胞,通过RT-PCR分析,证实细胞中存在ORF2基因的转录产物。
- Hep2 cells were transfected by the recombinant vector and apoptosis of the transfected cells was evaluated by trypan-blue exclusion and the agarosegel electrophoresis of DNA. 将该重组载体经阳离子脂质体转染入人喉癌细胞株Hep2中, 以台盼蓝拒染法和基因组DNA琼脂糖凝胶电泳, 观察转染的Hep2细胞的凋亡。
- The expression of B-RAF gene and Bcl-2 gene in BGC823 cells was detected by RT-PCR and the level of apoptosis was evaluated in transfected cells by flow cytometry. MTT检测胃癌BGC823细胞增殖情况; 流式细胞仪检测细胞凋亡情况,并与对照组进行比较。
- The toxic effects of 5-FC and GCV used alone or in combination on the transfected cells were observed by MTT assay, with the non-transfected RM-1 cells serving as control. 联合前药5-FC和/或GCV作用于转染了CD-TK基因的RM-1细胞,以MTT法检测自杀基因系统对转染后的RM-1细胞的杀伤作用,以未转染的RM-1细胞作对照。
- Specific bright yellow green flurescene was observed around the membrane and across the plasmas of the transfected cells when tested with anti-chicken IgY, FITC conjugate. 将重组疫苗转染 Hela细胞 ,用兔抗鸡 Ig Y进行间接免疫荧光试验 ,结果 ,在细胞膜和细胞浆中观察到了特异性的黄绿色荧光 ,证明表达产物具有特异性。
- Polymeric carrier containing folic acid is expected to be able to identify tumor surface receptors and transfect cells by receptor-mediated endocytosis. 含有叶酸的聚合载体能够识别肿瘤表面受体，并且能够通过受体介导的(细胞)内摄作用转染细胞。
- As SV40 plasmids replicate out of control in COS7 cells and be lethal to such transfected cells, SV40 vector/COS7 cell system has only been using as transient expression system for a long time. 基于猴病毒40(SV40)载体在COS7细胞内的复制没有节制并对细胞具有致死性,长期以来SV40载体-COS7系统只被用作瞬时表达。
- A study on the resistance of transfected cells with hSOD gene to superoxide a_nion_induced cytotoxicity caused by paraquat and xanthine (X)/xanthine oxidase (XO) is carried out in this paper. 研究了已获得稳定表达的hSOD基因导入细胞 (CMV -SOD细胞 )对百草枯 (paraquat)和黄嘌呤 (X) /黄嘌呤氧化酶 (XO)产生的超氧阴离子造成的细胞毒性的抵抗作用 .
- The results indicated that the enchancement of HLA-I heavy chain synthesis and TAPi gene mRNA expression in HBV transfected cells might mediate the up-regulation of HLA-I surface expression. 提示HBV诱导转染细胞内HLA一A基因重链合成量及1人P:基因转录水平增高，可上调细胞的HLA一I表面表达。
- The sensitivity of transfected cells to GCV and "bystander effect (BSE)" of HRE-TK/GCV system under normoxia or hypoxia conditions were measured by MTT assay and mixed coculture experiment. 用MTT法和混合共培养实验分别检测转基因细胞在缺氧或不缺氧状态下对GCV的敏感性以及HRE-TK/GCV系统的“旁观者效应”;
- After being transfected with antisense SLP-2, the expression of SLP-2 mRNA was declined 50%. The transfected cells showed slower growth, and the number of cells in G_1 phase was significantly increased by 10.5%, and S phase was declined by 9.8%. 反义质粒转染后SLP-2mRNA表达水平减少50%25;细胞生长变慢;其中G1期增高10.;5%25;S期减少9
- The vector pcDNA3.1-hVE GF165 was transfected into BMMSC with the method of liposome mediated. The expre ssion of human VEGF165 in the transfected cells was detected by RT-PCR,ELISA a nd Western blotting analysis. 用脂质体介导pcDNA3.;1-hVEGF165转染BMMSC;观察转染后细胞形态和生长情况的变化;通过RT-PCR、Western和ELISA鉴定VEGF在细胞中的表达情况
- NIH/3T3 cells and human gastric cancer cell line 823 were cotransfected with c-myc antisense RNA expression plasmid and pSV_2neo plasmid by electroporation method. Transfected cell lines selected by G418 were obtained. 采用c-myc反义RNA表达质粒与pSV_2neo质粒经电击穿孔技术共转染了NIH/3T3细胞和人胃癌823细胞,经适当浓度G418筛选后,获得其相应的转染细胞株。
- The expression of E cadherin was higher in SHP 2C>S GFP transfected cell lines than that in SHP 2 GFP transfected cell lines, while the expression of MMP 1 and MMP 9 was higher in SHP 2 GFP transfected cell lines. 免疫印迹结果表明 ;SHP 2C >S GFP细胞的E 钙粘蛋白表达比SHP 2 GFP细胞明显升高 (P <0 0 5 ) .;MMP 1及MMP 9的表达量在SHP 2 GFP细胞中有所增强 (P <0 0 5 )
- Bright green fluorescence of the transfected cells could be observed underconfocal after 24h of transfection. 转染后24小时,激光共聚焦扫描显微镜下可以观察到明亮的绿色荧光。
