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- The signal was intensified by increasing the number of fluorophores labeled on the target DNA segment. 两种引物分别命名为:多标记线性引物和多标记分支引物。
- Oligonucleotide-directed site-specific mutagenesis is previously used routinely to introduce desired mutations into target DNA sequences. 它不仅可以阐明基因表达的调控机理,还可用研究蛋白质结构与功能间的关系,改造天然蛋白质,使之更符合应用需求。
- Apparatus and methods are described for detecting target DNA in a biological sample using capture probes and electrically-assisted hybridization. 本发明描述了利用捕获探针和电子辅助杂交来检测生物样本中的目标DNA的装置和方法。
- The acquirement of the target DNA fragment was important and necessary for the preparation and detection of the probe, which was about to guarantee the specifity of the probe. 该基因的获得及鉴定为探针的制备及检测提供了必需的材料,并保证了探针的特异性。
- Methods: The serum samples were collected from 56 patients with morbilliform maculopapular eruption.The target DNA of CBV was detected by RT-PCR and restriction enzyme analysis. 方法:取56例春季流行性麻疹样发疹患者外周血标本,扩增目的DNA并进行特定限制性内切酶分析。
- Other binding modes also occur, depending on the target DNA sequence and on how we modify the PNA's bases.Of these, double duplex invasion is particularly interesting. 如果目标DNA序列以及PNA分子上碱基有所修改,我们还可观察到其他结合模式,其中双重双股螺旋入侵格外有趣。
- The target DNA segment was amplified by polymerase chain reaction.The PCR products were either used for direct sequence or ligated to PCR II vector for sequence. 用PCR方法将靶目标DNA扩增后直接用于序列测定或连接到PCR II载体后进行序列测定。
- PCR requires the synthesis of oligonucleotide primers complementary to the target DNA. The entire sequence of the target DNA does not need to be known, only the flanking regions. 就整个PCR扩增技术而言,引物的设计占有十分重要的地位,它的优劣直接关系到PCR的特异性与成功与否。
- Methods The target DNA fragments of BDV p40 gene was obtained by PCR amplification and inserted into pEGFP-N1 reporter vector. The constructed recombinant plasmid was confirmed by PCR and DNA sequencing. 方法通过PCR方法扩增获得博尔纳病病毒p40基因的完整序列,将此片段定向克隆到pEGFP-N1载体多克隆位点区,筛选重组阳性菌株,提取重组质粒,利用PCR方法和核酸序列测定验证重组质粒构建的正确性。
- Aim To investigate the cytotoxic activity of HDAC inhibitor Trichostatin A (TSA)combined with anticancer drugs targeting DNA on T24 bladder cancer cell line. 目的观察HDAC抑制剂曲古霉素A(TSA)和针对DNA的抗癌药物联用对膀胱癌T24细胞的杀伤作用。
- Bacillus spp. target DNA sequences 芽孢杆菌靶序列
- Plaque assays were done to isolate those white recombinant clones for rhBMP2 and 3. PCR analyses of viral DNAs were done to identify the target DNAs in recombinant viruses . 空斑形成后,经X-gal染色,挑取白色的单个重组克隆保存,取B2和B3各5个白色克隆进行第一轮扩增,收集病毒上清。
- The torpedo homed in on its target. 鱼雷射向目标。
- The company has met their export target. 该公司已经实现了它们的出口目标。
- A stationary target is easiest to aim at. 静止的目标是最容易瞄准的。
- He shot an arrow, but it fell short of the target. 他射了一箭,但没有射中靶。
- He won't hit the target at that distance. 他打不中那样远的目标的。
- Shots were fired at the enemy target. 有人向敌军目标开枪。
- DNA fingerprinting can help the police to find the criminals. DNA指纹术有助於警察发现罪犯。
- You missed the target by a mile/by miles. 你远未中的。