Plaque assays were done to isolate those white recombinant clones for rhBMP2 and 3. PCR analyses of viral DNAs were done to identify the target DNAs in recombinant viruses .

 
  • 空斑形成后,经X-gal染色,挑取白色的单个重组克隆保存,取B2和B3各5个白色克隆进行第一轮扩增,收集病毒上清。
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