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- Recombinant clone can be analyzed directly with the use of PCR.It is very efficient,especially in establishment of subtractive library of cDNA. 筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
- Objective To clone toxin_coregulated pilus subunit A (tcpA) gene of vibrio cholerae O139 strain XJ93006 and construct the recombinant cloning vector of pNEB 193_tcpA. 目的 以新疆分离 0 13 9霍乱弧菌XJ93 0 0 6株的DNA为模板 ,自行设计引物克隆毒素协同调节菌毛亚单位A(tcpA)基因 (包括侧序列 )并构建重组载体。
- Plaque assays were done to isolate those white recombinant clones for rhBMP2 and 3. PCR analyses of viral DNAs were done to identify the target DNAs in recombinant viruses . 空斑形成后，经X-gal染色，挑取白色的单个重组克隆保存，取B2和B3各5个白色克隆进行第一轮扩增，收集病毒上清。
- A Rapid Method of Using Oligonucleotide Probe to Screen Recombinant Clone 利用寡核苷酸探针快速筛选重组克隆
- This non sequitur invalidates his argument. 他不根据前提推理因而论证无效。
- Patience is a sine qua non for a good teacher. 做个优秀教师必不可少的条件是要有耐心。
- The diplomat was declared persona non grata. 这位外交家被宣布为不受欢迎的使节。
- He is declared personal non grata and forced to leave the country. 他被宣布为不受欢迎的人而被迫离开该国。
- recombinant clone 重组子克隆(分)
- I had had a few beers and was completely non compos mentis. 我喝了些啤酒就头昏脑胀了。
- Screen for recombinant clones by colony Polymerase chain reaction 应用以菌落为模板的聚合酶链反应技术筛选重组阳性克隆
- He was declared persona non grata and forced to leave the country. 他被宣布为不受欢迎的人而被迫离开该国。
- Wait for foster mother to give birth to the clone. 等待“养母”产下克隆幼仔。
- Will 2003 be the year of the first human clone? 人类第一个克隆人会在2003年诞生吗?
- We decided to declare them personae non grata. 我们决定宣布他们为不受欢迎的人。
- He was accused of spying and declared non grata. 他被控从事间谍活动而被宣布为不受欢迎。
- The first clone farm has appeared in New Zealand. 第一家克隆农场在新西兰出现。
- Cum non sis, qui fueris, non esse,cur"velis vivere. “当你到了今日之我非复昔日之我的时候，就没有再要活下去的理由了”。
- Restaurant open to non residents. 餐厅对非住宿者开放.
- He's got no originality- he's just a Mozart clone. 他不具独创性，他只是莫札特的翻版。
