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- Keywords phytase gene（phyA gene） Cloning Expression vector; 植酸酶基因;克隆;表达载体;
- gene cloning expression 基因克隆表达
- This review introduced the structure 、biologic activities and their mechanism of SAK, SAK’s gene cloning and expression research concerned. 本文从SAK的结构、生物学特性及机理、基因克隆及表达方面的研究进展等方面进行了综述。
- Gene cloning, expression and immunological identification of a single chain antibody fragment 3D3 against Dengue type 3 virus Si Bingyin, Yang Peiying, Qin Ede, et al. 中华实验和临床病毒学杂志1998年第3期第12卷论著作者：司炳银杨佩英秦鄂德徐品芳于曼单位：100850北京军事医学科学院微生物流行病研究所...
- ACC Oxidase Gene Cloning and Sequence Analysis of Citrus aurantium L. 酸橙ACC氧化酶基因片段克隆及序列分析。
- This feature is favorable for gene cloning using transposon tagging strategy. 这个特性有利于转座因子作为分子标签克隆异源植物中的结构基因。
- In the paper, studies on chitinase of microbes are briefly reviewed, and the classification, characteristics, purification, gene clone and expression, and its application in practice were recommended. 摘要概述了有关微生物几丁质酶的研究情况，介绍了几丁质酶的分类、性质、分离纯化、基因克隆与表达以及在实际中的应用。
- The goal of this experiment is to explore the feasibility of high throughput (HTP) gene cloning, expression, protein purification, eventually for large-scale structural and functional analysis. 北京大学口腔医学院（魏世成）；
- Methods:To clone and express E7 gene of HPV16 with gene clone technique. To pruify E7 protein and inoculate mice. The reactivity of special E7 antibody in sera was detected by ELIAS to evaluate antigenicity of E7 protein. 方法 :应用基因克隆技术克隆、表达HPV16E7基因 ,纯化E7蛋白并免疫动物 ,用ELISA检测动物血清中的特异性E7抗体 ,进而评估E7蛋白的抗原性。
- Gene cloning provides a means of purifying and propagating specific DNA segments. 基因克隆化提供了一种纯化和扩增特定DNA片段的方法。
- ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis. 通过引物设计、PCR、基因克隆、测序,获得了10种鸢尾属植物和1个外类群种的ITS序列。
- This paper summarizes and illustrates the strategy of how to do EST analysis and gene cloning through bioinformatics. 本文通过实例综述了怎样运用生物信息资源进行EST筛选及其新基因克隆的策略。
- As the most efficient strategy in gene clone, positional cloning has been used widely in QTL cloning in rice. 图位克隆作为基因克隆的最有效手段, 在水稻QTL克隆方面取得了很大的进展。
- Abstract Development of biotechnique, especially molecular marker technique, lays the foundation for R gene cloning. 摘要 分子生物学技术特别是分子标记技术的的飞速发展，为蔬菜作物抗病基因的克隆奠定了基础。
- These results would be useful for interest gene cloning and marker-assisted selection for genetic improvement in Chinese jujube. 枣树高密度遗传连锁图谱的绘制和性状QTL定位为挖掘枣树有益基因打下了基础,为枣树分子遗传育种提供了依据。
- Objective： To screen for the gene clones with differential expression in normal liver and hepatoma. 目的 筛选肝癌与正常肝基因表达差异克隆。
- Dominating methods in wheat gene cloning include homology-based cloning, map-based cloning, suppression subtractive hybridization, DDRT-PCR etc. 目前，小麦基因的克隆方法主要有同源克隆、图位克隆、差减杂交、差异显示等。
- Here this article reviews the properties, gene cloning, structure and function of abrin, and its application in targeted cancer therapy. 本文就其性质、基因克隆、结构与功能,以及在肿瘤导向治疗方面的应用作一简要的综述。
- Conclusions:E7 protein of HPV16 (HB strain) could be prepared by gene clone technique.HB-E7 protein displayed antigenicity of standard E7 protein. 结论：HPV16湖北株E7蛋白可通过基因克隆方法制备，并保留了标准E7蛋白的抗原性。
- Objective] By sequencing of SSU rRNA gene cloning from Xinjiang cutaneous leishmaniasis pathogen (XJCLP) to provide evidence for identification of the pathogen. [目的 ]分析新疆皮肤利什曼病病原体SSUrRNA基因序列 ,为该病原体种株鉴定分析提供实验依据。
