Methods:To clone and express E7 gene of HPV16 with gene clone technique. To pruify E7 protein and inoculate mice. The reactivity of special E7 antibody in sera was detected by ELIAS to evaluate antigenicity of E7 protein.

• 方法 :应用基因克隆技术克隆、表达HPV16E7基因 ,纯化E7蛋白并免疫动物 ,用ELISA检测动物血清中的特异性E7抗体 ,进而评估E7蛋白的抗原性。