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- Objective:To construct an eukaryotic expressing vector pIRES1neo/hFL and express hFL in COS 7 cell. 目的 :构建人FL真核表达载体 pIRES1neo/hFL ,观察其在COS 7细胞中的表达。
- Pichia pastoris expressing system,which is a eukaryotic expressing system developed in recent years,has a promising prospect. 巴氏毕赤酵母表达体系是近年发展起来的真核表达体系,具有良好的应用前景。
- Objective To construct mouse granulocyte-macrophage colony stimulating factor (mGM-CSF) gene eukaryotic expressing plasmid pcDNA3-GM-CSF, to transfect the recombinant into erythroleukemia cell line FBL-3, and identify their biological activity. 目的 构建小鼠转粒细胞 巨噬细胞集落刺激因子 (mGM CSF)真核表达质粒 pcDNA3 GM CSF ,转染红白血病细胞系FBL 3,并鉴定其活性。
- Objective To construct the antisense nucleic acid eukaryotic expressing vector of the cDNA sequence encoding the transmembrane domain of receptor tyrosine kinase RON (RON-RTK) gene (RONm). 目的构建RON基因跨膜区段(RONm)的反义核酸真核表达载体。
- The recombinant eukaryotic expression plasmid,pcDNA3.1A DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. 成功构建重组真核表达质粒 pcDNA3 .;1A DCN;转染MsC并筛选出 2个阳性克隆株。
- The eukaryotic expressing vector encoding human cbl is constructed and it expresses flag-cbl fused protein correctly in COS-7 cells. 成功构建了N 末端带flag标签的cbl真核表达载体 ;并使其在真核细胞COS 7中表达 .
- Objective To construct endothelial cell-specific molecular-1(ESM-1) eukaryotic express vector,transfect it into human umbilical vein endothelial cells( HUVECs,ECV304) and express it in vitro . 目的 构建人内皮细胞特异性分子 1(ESM 1)真核表达载体 ,转染人脐静脉内皮细胞系ECV30 4 ,并在ECV30 4中获得表达。
- Results The bicistronic eukaryotic expression plasmid was corrected and can co - express human BMP -2 and VEGF165 mRNA in vitro. 该重组质粒能在体外同时表达BMP2及VEGF165 mRNA。
- Objective:To construct and express a recombinant eukaryotic expression vector bearing fusion gene of human IL-2 cDNA gene and Fc fragment. 目的 :构建含人IL 2cDNA基因和免疫球蛋白Fc片段融合基因的真核表达载体pcDNA 3.;1IL 2 /Fc;并在真核细胞中表达;以期用于乙型肝炎病毒 (HBV)DNA疫苗的研究。
- AIM: To clone human IL-24 (hIL-24) gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL-24 protein. 目的:克隆人IL-24基因并构建真核表达载体;转染Ca Ski细胞进行真核表达.
- CB could be expressed in high yield by vaccinia virus eukaryotic expression system. 痘苗病毒真核表达系统可以高效表达目的蛋白。
- Eukaryotic expression vector pCDNA3-AADC can be efficiently expressed in primary muscle cells. 含人类神经元AADC基因的真核表达重组体可在原代培养的骨骼肌细胞中有效的表达。
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。
- The constructed eukaryotic expression vector contained gB680 and pp65m fusion gene and expressed in COS-7 cells. 重组质粒含有gB680和pp65m融合基因,并能在COS-7细胞中表达。
- Abstract: Objective To construct the eukaryotic expression vector which express the protein of VZV glycoprotein E in COS7 cells. [摘 要] 目的 构建水痘-带状疱疹病毒（VZV）糖蛋白E的真核表达载体并使其在COS7细胞中表达。
- Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid. 目的克隆人血管紧张素转换酶2基因(ACE2),并构建其真核表达载体。
- AIM: To clone human soluble TRAIL cDNA and construct its eukaryotic expression vector to prepare a condition for targeted gene therapy. 克隆人可溶性trail(strail)基因并构建肿瘤细胞特异性真核表达载体;为肿瘤基因治疗的靶向性奠定实验基础.
- Recombinant eukaryotic expression vector pCMV-Script/mito-MPG was constructed by molecule-cloning technique, and then transfected into A549/DDP cells. 构建pCMV-Script/mito-MPG重组真核表达载体; 脂质体将其转染至人非小细胞肺癌多药耐药细胞A549/DDP;
- Objective To construct a eukaryotic expression vector for expression of human neuropathy target esterase(NTE) esterase domain in mammalian cells. 目的构建人神经病变靶标酯酶(NTE)酯酶活力域(NESP)真核表达载体,并在细胞中表达。
- AIM: To clone rat CYP2J3 gene, construct Pcdna3.1(+)-CYP2J3 eukaryotic expression vector and detect its expression in endothelial cells of bovines. 目的:克隆大鼠CYP2J3基因并构建Pcdna3.;1(+)-CYP2J3真核表达载体;检测其在牛内皮细胞中的表达。
