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constitutive shuttle vector 组成型穿梭表达载体
We also have constructed two shuttle vector plw9,psc11m1 which can recombine to FPV genome. 我们还构建了可以和FPV基因组发生重组的穿梭载体，plw9和psc11。
The Pseudorabies virus (PRV) Fa strain gC gene was subcloned into Adenovirus shuttle vector pDC316 through double enzymatic digestion. 通过双酶切将伪狂犬病毒(Pseudorabies virus,PRV)Fa株gC囊膜糖蛋白基因片段亚克隆到5型腺病毒AdMax系统穿梭质粒pDC316中,得到pDC316-gC。
The recombinant shuttle vector pBacPAK-CTB and pBacPAK-4aa-CTB was co-infected with Bm-BacPAK6 DNA into BmN cells. The recombinant virus was screened, plaque-purified and identified by PCR and Southern hybridization. 重组转移载体DNA与经Bsu36I酶切线性化的修饰型病毒Bm-BacPAK6 DNA共转染家蚕BmN细胞，然后经空斑纯化、PCR扩增、Southern杂交等方法鉴定出含CTB基因的重组病毒rBm-BacCTB和rBm-Bac4aa-CTB。
As the pMCLacI/neo shuttle vector is a novel bi-functional mutation detection system, apart from be used in above transgenic animal study , it was also used in our study of MNNG-indued DNA damage and repair research. 由于本研究率先在突变检测系统中应用了具有双功能的pMCLacI/neo穿梭载体,在利用转基因动物技术建立突变检测系统及展开相关研究的同时，我们又利用基因敲除技术，定向敲除DNA损伤修复酶基因-MGMT，建立了一系列MGMT基因定向敲除的突变检测系统，及高表达MGMT的突变检测系统，并展开了相应的DNA损伤修复的研究。
The target fragments were cut down from pGEMT-easy and ligated with shuttle vector pJFF224-XN/E. coRI. Through identification, the homogenous regulatory gene inserted in ?cis? and ?trans? direction under T4 promoter, separetely. 切下目的片段与酶切穿梭质粒pJFF224-XN连接,经PCR及酶切鉴定,调节基因片段分别以正向或反向插入到质粒的T4启动子下。
The target fragments were cut down from pGEMT-easy and ligated with shuttle vector pJFF224-XN/E. coRI. Through identification, the homogenous regulatory gene inserted in cis and trans direction under T4 promoter, separately. 切下目的片段与酶切的穿梭质粒pJFF224-XN连接,经PCR方法及酶切鉴定,调节基因片段分别以正向或反向插入到质粒的T4启动子下;
Then, the DNA fragment xylR-gfp which were obtained after pGEM-xylRGFP being digested by Kpn I and Sph I , was inserted into Kcoli-B.subtilis shuttle vector pRP22, and the resulted recombinant plasmid was named at pRP22-GFP. 经SphI/KpnI双切质粒pGEM-xylRGFP后，回收片段xylR-gfp，插入穿梭载体pRP22，得到重组子pRP22-GFP。
E. coli-B. subtilis shuttle vector 穿梭载体
E.coli-mycobacterium shuttle vector 大肠杆菌-分枝杆菌穿梭质粒
The strain BSG is more efficient recipient of transformation for shuttle vectors pPTE4 and pBE2 from E. 来自大肠杆菌的穿梭质粒在BSG菌中转化频率较亲本高。
Construction of an EB Virus Based Shuttle Vector Plasmid pZH32 穿梭质粒pZH32的构建
Construction and Optimization of E. Coli and B.Subtilis Shuttle Vector 大肠杆菌枯草杆菌穿梭载体的构建及优化
The U.S. made the first space shuttle in the world. 美国制造了世界上第一架航天飞机。
The space shuttle will orbit a weather satellite. 太空梭将把一个气象卫星送入运行轨道。
I'm flying to Boston on the shuttle. 我将乘穿梭班机去波士顿。
The buses shuttle visitors to Disneyland. 区间公共汽车将游客载至迪尼斯乐园。
Construction of Shuttle Vector by Using Thya Gene as a Selective Pressure 以胸苷酸合成酶基因为选择压力的穿梭质粒载体的构建
The shuttle moves backwards and forwards through the warp. 梭子在经线中来回移动。
Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题：图1.;穿梭表达载体pRL_hEGF的构建。

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