Then, the DNA fragment xylR-gfp which were obtained after pGEM-xylRGFP being digested by Kpn I and Sph I , was inserted into Kcoli-B.subtilis shuttle vector pRP22, and the resulted recombinant plasmid was named at pRP22-GFP.

• 经SphI/KpnI双切质粒pGEM-xylRGFP后，回收片段xylR-gfp，插入穿梭载体pRP22，得到重组子pRP22-GFP。