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- cDNA cloning vectors 表达载体构建
- These are inserted into a cloning vector. 它们被插入克隆载体。
- The TuMV isolate CDN1 infectious full-length cDNA clone pVIR95T was modified into an expression vector pVIR95TM by inserting the adaptor with the MCS and the new NIa recognition site after the site between the Nib and CP by PCR. 在此基础上，将含有多克隆位点和编码NIa蛋白酶识别位点的Adaptor通过PCR介导插入到芜菁花叶病毒CDN1株系全长cDNA的NIb与CP区之间的NIa蛋白酶识别位点之后，将芜菁花叶病毒CDN1株系全长cDNA侵染性克隆pVIR95T改造成一个可利用的基因插入型表达载体pVIR95TM。
- CDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province. 云南省烟草花叶病毒和黄瓜花叶病毒外壳蛋白基因克隆及序列分析。
- We introduced the cDNA clone of ARV-2 from USA in 1988. We subcloned this full-length cDNA into pUC19 by Kpn I site. 艾滋病相关病毒(ARV-2)是目前应用最广的艾滋病毒分离株之一.
- Methods:APAF-1 cDNA types in leukemic cell lines K562 and CEM/VLB100 were studied by cDNA cloning strategy. 方法:应用RT-PCR结合测序方法研究白血病细胞系K562和CEM/VLB100APAF-1cDNA类型;
- In GENETIC ENGNEERING, nonviral DNA can be inserted into a phage, which is then used as a cloning vector. 在基因工程中，没有病毒的DNA可以被插入到噬菌体中，用作克隆载体。
- A cDNA clone of 1 629 bp was obtained by screening the library with an adult stage specific probe. 利用消减杂交 (subtractedhybridization)获得的旋毛虫成虫期特异性探针筛选成虫cDNA文库 ,获得一个长 16 2 9bp的基因。
- An outline of the procedure for introducing a piece of foreign DNA into a cloning vector is shown in Figure 1. 3. 图1.;3列出了引出一段外源DNA进入克隆载体的大概步骤。
- Objective To construct the cDNA subclones spanning the entire genome of dengue 2 virus NGC strain for further construction of full-length infectious viral cDNA clone. 目的构建登革2型病毒NGC株基因组全序列的亚cDNA克隆,为进一步构建全长感染性cDNA克隆奠定基础。
- A cDNA clone encoding a pheromone binding protein 3 from antenna of Helicoverpa assulta (named HassPBP3) was isolated by reverse transcription polymerase chain reaction (RT-PCR). 利用RT-PCR技术从烟实夜蛾Helicoverpa assulta( Hass)雄虫触角中扩增得到了信息素结合蛋白3( HassPBP3)。
- Objective To clone toxin_coregulated pilus subunit A (tcpA) gene of vibrio cholerae O139 strain XJ93006 and construct the recombinant cloning vector of pNEB 193_tcpA. 目的 以新疆分离 0 13 9霍乱弧菌XJ93 0 0 6株的DNA为模板 ,自行设计引物克隆毒素协同调节菌毛亚单位A(tcpA)基因 (包括侧序列 )并构建重组载体。
- The cDNA Cloning of Pokeweed Antiviral Protein Gene and Research on Gene-transforming Tobacco[J].Journal of Henan Agricultural University,2006,40(2):130-132,141. PAP基因cDNA克隆及烟草转基因研究[J].;河南农业大学学报;2006;40(2):130-132;141
- Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants, it has been used as a cloning vector in GNETIC ENGINEERING. 在许多双子叶植物中质粒在寄主细胞中是独立的复制单元，所以可以在基因工程中用作克隆载体。
- Methods Full length HMGB1 cDNA cloned by PCR from mammary library was inserted into pET28a(+) vectors to construct pET28a(+)-HMGB1 plasmid. After confirmation, the positive pET28a(+)-HMGB1 recombinant plasmid was transformed into E. 方法应用PCR从乳腺文库中扩增得到HMGB1基因片段,并将其克隆到pET28a(+)载体上,对获得的pET28a(+)-HMGB1重组质粒扩增后进行PCR、酶切和测序鉴定。
- The full-length cDNA clone, NDV3GM122, and the three helperplasmids pCI-NP, pCI-P and pCI-L of Newcastle disease virus strain ZJI isolated from an outbreak in the goose were cotransfected into BSR-T7/5 cell expressing T7 RNA polymerase. 利用反向遗传操作技术,将ZJI株鹅源新城疫病毒全基因组cNDA克隆(NDV3GM122)和含该毒株NP、P及L基因的3个表达载体(pCI-NP、pCI-P与pCI-L)共转染BSR-T7/5细胞;
- Forty-four cDNA clones, representing 44 defense-responsive genes, were fine mapped to 56 loci distributed on 9 of the 12 rice chromosomes. 44个cDNA克隆代表44个抗病相关基因被定位于水稻12条染色体中的9条染色体上的56个位点。
- full-length genomic cDNA clone of Gx strain Gx株全长cDNA
- The PCR products were cloned into T clone vector,and the positive clones were picked out,then the T clone vector was identified by restriction endonuclease digestion. 将扩增的连接产物克隆入T载体，挑选阳性克隆并进行酶切鉴定，酶切鉴定正确的克隆进行拼接产物的核苷酸序列测序。
- The availability of cDNA clones for EcR and USP from several insect species has opened up possibilities for the development of high throughput EcR/USP based screening assays. 多种昆虫蜕皮激素受体和超气门蛋白基因的成功克隆为建立基于昆虫蜕皮激素受体的高通量筛选技术奠定了基础，并且可以通过多种途径实现。
