The PCR products were cloned into T clone vector,and the positive clones were picked out,then the T clone vector was identified by restriction endonuclease digestion.

• 将扩增的连接产物克隆入T载体，挑选阳性克隆并进行酶切鉴定，酶切鉴定正确的克隆进行拼接产物的核苷酸序列测序。