您要查找的是不是:
- Objective:To illustrate the effect on myeloma cells with TK gene by GCV. 目的:探讨更昔洛韦对转染TK基因的骨髓瘤细胞生长的抑制作用。
- The ovarian cancer cell line transfered by HSV TK gene regressed rapidly in response to the GCV therapy. AO细胞转染HSV-TK基因后,能有效地被GCV杀灭。
- LacZ gene was inserted into TK gene deleted transfer vector, the TK /LacZ+ /GTPV-TY mutant was screened. 将LacZ基因表达盒插入TK基因缺失转移载体,筛选TK基因缺失的GTPV-TY突变株。
- In this assay,we studied the possibility of raccoon pox virus (RCN) TK gene as a viral selection marker on Rat-2 cells. 本文在Rat-2(TK~-)细胞上,对浣熊痘病毒TK基因作为病毒筛选标记的作用进行了研究。
- Tumor cells expressing HSV tk gene can be eradicated by administration of GCV in vitro as well as in vivo. 在invitro和invivo水平,表达tk基因的肿瘤细胞均被GCV有效杀伤,这表明HSV-tk/GCV自杀基因系统有可能成为基因治疗脑瘤的有效方法。
- Objective To test the anti-tumor effect of adenovirus vector generated from the homologous recombination of bacteria mediating TK gene on hepatic neoplasm cell. 目的探讨用细菌内同源重组法制备含TK自杀基因腺病毒对肝癌细胞的杀伤作用。
- Objective To observe the sensitivity of pulmonary adenocarcinoma cell A549 transfected with TK gene to acyclovir ACV in nude mice. 目的 观察活体内转TK基因肺腺癌细胞对ACV(无环鸟苷 )的敏感性。
- According experiment in vitro , it is proved that when HSV TK gene is transferred by STK, the sensitivity of GCV to Psi2STK is improved. 通过体外实验证实由逆转录病毒载体 ( STK)传导的 HSV- TK基因 ,在培养的 C6胶质瘤细胞中 ,可提高 GCV对 psi2 STK细胞的敏感性
- Methods:By using RV HSV TK gene system and MTT technique, the growth inhibited effect on myeloma cells with TK gene by GCV were analysed. 方法:应用缺陷型反转录病毒介导的TK基因系统(RV-HSV-TK)转染骨髓瘤细胞,采用细胞增生实验(MTT法),研究更昔洛韦对转染TK基因的骨髓瘤细胞株的抑制作用。
- The TK gene fragment (1.36Kb) of the six APV strains was amplified by polymerase chain reactioin (PCR) using the primers H1+ and H2-. 通过PCR方法用自行设计的一对引物H1+\\H2-扩增出了四个鸽痘病毒分离株、鸡痘病毒疫苗株和鸽痘病毒疫苗株的TK基因片段,长度约为1.;36Kb。
- The genes that flank the TK gene in the six APV strains had 15bp directed repeats and 8bp inverted repeats. 各毒株TK基因的侧翼都存在15bp的正向重复序列.;8bp的倒转重复序列;
- Objective To study the killing effect of suicide gene TK to murine gastric cancer and observe the role of immune response in enhancing TK killing capacity following TK gene combined with mGM-CSF gene. 目的 观察自杀基因TK对小鼠胃癌的杀伤作用 ,并联合mGM CSF基因 ,观察免疫反应在增强TK杀伤性中的作用。
- Objective: To amplify the CD and TK gene fragment and analyze the DNA sequence,and then to construct eukaryotic expression plasmids pIRES-CD and pIRES-TK and determine their expression in ACC-2 cells. 目的:运用分子生物学技术扩增CD、TK基因,进行其DNA序列分析,并构建真核表达质粒pIRES-CD及pIRES-TK,将其共同转染ACC-2细胞,为进一步研究双自杀基因对ACC-2细胞杀伤作用奠定基础。
- Objective: To assess the treatment effect of rat C 6 glioma with recombinant adenovirus HSV tk gene (AdHCMV tk) combinated with GCV and ACV. Methods: The efficiency of AdHCMV lacZ infecting C 6 cells was studied by using x gal staining. 目的:带有HSV-tk基因的重组腺病毒(AdHCMV-tk)结合核苷类似物(NA)治疗大鼠C6脑胶质瘤。
- Results The HSV TK vector producer cell line GRC 1/TK was obtained which confered a chemosensitivity to ACV. Conclusions HSV1 TK gene might be used in gene therapy of human renal cancer GRC 1 cells. 结论 HSV1 TK基因可成功转染人肾癌GRC 1细胞 ,为基因治疗肾癌提供实验依据。
- Objective:To illustrate the effect on myeloma cells with TK gene by GCV.Methods:By using RV HSV TK gene system and MTT technique, the growth inhibited effect on myeloma cells with TK gene by GCV were analysed. 目的 :探讨更昔洛韦对转染 TK基因的骨髓瘤细胞生长的抑制作用。 方法 :应用缺陷型反转录病毒介导的 TK基因系统 (RV- HSV- TK)转染骨髓瘤细胞 ,采用细胞增生实验 (MTT法 ) ,研究更昔洛韦对转染 TK基因的骨髓瘤细胞株的抑制作用。
- The recombinant adenovirus containing rPB-DR promoter and TK gene has been constructed successfully, and this lays the foundation for the research of gene therapy of prostate cancer . 成功构建出包含rPB DR启动子和TK自杀基因的重组复制缺陷型腺病毒并鉴定正确,可用于下一步前列腺癌基因治疗的研究中。
- TK gene was 534bp and encoded 178 amino acids. On the N terminal of TK, ATP motif was found. TK gene was conserved in CPV, the identity of GTPV-TY TK gene compared with TK gene in other CPV strains were more than 96%. 试验中首先用PCR方法克隆了GTPV-TY的TK基因,TK基因ORF大小为534bp,编码178个氨基酸,TK的N端具有ATP结合结构域,与其它毒株的TK基因的核苷酸序列比较显示CPV各毒株TK基因的同源性大于96%25,保守性很高。
- TK-gene system (retrovirus transfer of the herpes simplex virus thymidine kinase gene) is one of the much more mature plans. We have succeeded in transmitting herpes TK gene into human pancreatic cancer cells, named SW1990 by retrovirus. 由缺陷型逆转录病毒介导的TK基因系统(RV-HSV-TK)是众多肿瘤生物治疗方法中一个技术较为成熟的方案,本实验采用该系统在体外成功地转染了人胰腺癌细胞株SW1990并能够稳定传代培养,转染了TK基因的SW1990细胞(SW±K),其生长曲线与未转染的SW1990细胞无差异。
- Methods HSV TK gene was transfected to alimentary carcinoma cell lines (LOVO, MGL 803 and Bel 7402) mediated by recombinant adenovirus. Efficiency of transfection between each cell line was compared. 方法腺病毒介导的自杀基因HSV-TK分别转染结肠癌LOVO细胞、胃癌MGL-803细胞和肝癌BEL-7402细胞,比较腺病毒载体对不同肿瘤细胞的转染效率。