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- Methods RT polymerase chain reaction(RT PCR), immunohistochemical methods and in situ hybridization technologies were used to detect the PCNA, L faabp mRNA level and located distribution at embryonic period and natal period. 方法利用免疫组织化学、原位杂交和反转录聚合酶链反应(RT-PCR)等技术,分别检测胚胎期E14d,E17d,E19d和幼鼠期P1d,P2d,P7d,P28d大鼠小肠上皮细胞增殖细胞核抗原(PCNA)、L-fabpmRN水平及其定位分布。
- Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR). 方法采用逆转录聚合酶链反应（RT－PCR）检测84例静脉毒瘾者血浆标本。
- METHODS The reverse transcription polymerase chain reaction(RT PCR) was used to detect expression of mdr 1 mRNA in 20 RCC and 5 normal kidneys. 方法应用逆转录?聚合酶链反应（RT?PCR）技术检测20例肾癌和5例正常肾组织mdr?1基因mRNA的表达。
- Method:By using reverse transcriptase polymerase chain reaction (RT PCR), S, mdr 1 and LRP gene expression were studied in 46 de novo AML cases. 方法 :应用RT PCR法检测 4 6例初治AML患者的S、mdr 1和LRPmRNA表达的阳性率。
- Method:The HEp 2 cells after elemene treatment were analyzed utilizing Westernblot and reverse transcriptase polymerase chain reaction (RT PCR). 方法 :使用比色法 ,研究榄香烯诱导人喉鳞状细胞癌细胞株HEp 2细胞凋亡时Caspase 3活性的变化 ;
- The genome of NS5A and HVR1 region were amplified by the methods of Reverse Transcription Polymerase Chain Reaction ( RT - PCR). HCV HVR1及NS5A基因片断扩增：采用巢式逆转录聚合酶链反应法(RT-PCR)。
- Methods Using reverse transcription polymerase chain reaction (RT PCR) technique, we determined the levels of mdr 1 mRNA in 82 breast cancer samples. 方法采用逆转录－多聚酶链式反应技术，检测了82例次乳癌组织的mdr－1mRNA水平。
- Methods:Reverse transcription polymerase chain reaction (RT PCR) was used for determination of EMT expression in tumor cell lines. 方法：应用反转录多聚酶链反应（RT?PCR） 检测EMT 在肿瘤细胞系的表达。
- The difference of bone morphogenic protein 2 (BMP 2) mRNA expression in hMSC and osteoblasts was compared by reverse transcriptase polymerase chain reaction(RT PCR). RT-PCR法比较骨形成蛋白(BMP)-2mRNA在hMSC和成骨细胞中的表达差异。
- METHODS Using reverse transcription polymerase chain reaction (RT PCR),the expression of CaM mRNA was measured in HPAA of rats which were injected with acetic acid cortisone. 方法 以逆转录多聚酶链反应 (RT PCR)半定量法测定大鼠注射醋酸可的松后钙调素 (calmodulin ,CaM)mRNA的表达水平。
- Methods:M bcr/abl mRNA was assayed by reverse transcriptase polymerase chain reaction (RT PCR) in bone marrow cells from 46 successfully sibling marrow engrafted CML patients. 方法：用逆转录－多聚酶链反应（RT－PCR）技术测定46例经alo－BMT植活的CML患者骨髓细胞M－bcr/ablmRNA表达。
- For his invention of the polymerase chain reaction (PCR) method. (聚合酶链反应法的发明)。
- Objective To develop a rapid and simple method of reverse transcription polymerase chain reaction (RT PCR) for the detection of mRNA from human cytomegalovirus (HCMV) immediate early (IE) gene. 目的建立快速简便的逆转录聚合酶链反应（RT?PCR）方法检测人巨细胞病毒（HCMV）即刻早期（IE）基因mRNA。
- Reverse transcription polymerase chain reaction (RT PCR) assay was used to detect the expression of MDR 1 mRNA and MRP mRNA in 35 patients. Meantime, Immunohistochemistry SABC technique was used to detect P glycoprotein(P gp) in 61 cases. 应用逆转录－多聚酶链式反应（RT－PCR）技术，检测了35 例喉癌患者MDR－1 m RNA、MRPm RNA 基因的表达，同时，应用免疫组化SABC法检测了61 例患者MDR－1 基因产物P－gp。
- In order to study the effect of iron on the expression of UCP2 in white fat and UCP3 in muscle in obese rats, a reverse transcription polymerase chain reaction (RT PCR) technique is used to measure the expression of UCP2 and UCP3 mRNA. 为了探讨铁对肥胖大鼠白色脂肪组织中UCP2及骨骼肌中UCP3基因表达的影响 ,应用RT PCR方法测定UCP2、UCP3mRNA表达水平。
- Methods Anti HGV, HBsAg, anti HBs, anti HBc and anti HCV were detected by enzyme linked immunoassys (EIA). Anti HGV positive sera were further tested for HGV RNA by a nested reverse transcription polymerase chain reaction (RT nPCR). 方法应用酶联免疫法（EIA）检测抗?HGV、HBsAg、抗?HBs、抗?HBc及抗?HCV，并对抗?HGV阳性者进一步用逆转录套式PCR（RT?nPCR）法检测HGVRNA。
- Methods Five CML patients,2 in chronic phase(CP),1 in accelerated phase(AP) and 2 in blast crisis(BC), all comfirmed the presence of b3a2 bcr/abl mRNA by RT PCR(reverse transcriptase polymerase chain reaction). 方法 5例CML中慢性期 2例 ,加速期 1例 ,急变期 2例 ,均具b3a2型bcr/ablmRNA。
- Methods The expression of MDR1 gene in 77 cases of AL patients and 10 cases of control group was detected with semi quantitative reverse transcription polymerase chain reaction (RT PCR). 方法 采用半定量逆转录聚合酶链反应（ R T? P C R），对77 例 A L病人及 10 例正常人 M D R1 基因表达进行检测。
- Methods The levels of p73 transcripts in 61 ALL cell lines and 53 childhood ALL patients were assayed using reverse transcriptase polymerase chain reaction (RT PCR), and their relationship with the clinicopathological characteristics was analyzed. 方法 应用RT PCR技术检测 61个ALL细胞系和 53例儿童ALL患者的p73基因mRNA表达情况 ,并结合患者的临床资料进行分析。
- Methods To use specific serosorbernt templat polymerase chain reaction(SSST. 方法应用特异血清吸附模板聚合酶链反应(SSST。
