Methods RT polymerase chain reaction(RT PCR), immunohistochemical methods and in situ hybridization technologies were used to detect the PCNA, L faabp mRNA level and located distribution at embryonic period and natal period.

• 方法利用免疫组织化学、原位杂交和反转录聚合酶链反应(RT-PCR)等技术,分别检测胚胎期E14d,E17d,E19d和幼鼠期P1d,P2d,P7d,P28d大鼠小肠上皮细胞增殖细胞核抗原(PCNA)、L-fabpmRN水平及其定位分布。