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- ATF-lys10 mediated gene delivery is uPAR targeting, and the preparation of gene delivery complex is relatively simple. ATF-lys10介导的基因转移具有uPAR靶向性，而且基因转移复合物制备相对比较简单。
- Targeted gene replacement (TGR) is an important technique for gene function analysis. 目标基因替换是基因功能分析的重要手段。
- Stable Integration of Genomic Gene of Interest in Mammalian Cells Following Adeno-associated Virus Vector Mediated Gene Transfer. 腺相关病毒介导基因组来源的基因转移与整合。
- Objective To study the mechanism of bystander effect in herpes simplex virus thymidine kinase(HSV TK)/Ganciclovir(GCV) mediated gene therapy. 目的 探讨用单纯疱疹病毒胸腺嘧啶核苷激酶（HSV?TK）/丙氧鸟苷（GCV） 系统进行基因治疗时发生的旁观者效应机制。
- And LipofectamineTM 2000 mediated gene transfection of p498 siRNA expression vector into COS-7 cells downregulated the mRNA and protein level of 4-1BB at 48h after transfection. 结果经测序分析;各m4-1BB siRNA表达载体构建成功; 其中p498 siRNA与p4-1BB双质粒转染COS-7细胞;48h后可观察到细胞表面4-1BB分子明显下调;其胞内mRNA水平也显著降低;与无关干扰组比较;差异有统计学意义(P<0.;05)。
- Abstract: To improve the efficiency of targeted gene replacement (TGR), a dual screen (DS) system with gusA gene as negative selective marker (GUS-DS) was developed in Magnaporthe oryzae. 摘 要: 目标基因替换是基因功能研究的重要方法, 在生物工程领域广泛应用。
- Gene therapy is a synthetically technique of physics, chemistry, biology, and medical science.It is applied in gene replacement, gene correction, gene augmentation, gene inactivation. 摘要基因治疗综合了物理、化学、生物、医学等多门学科的技术，应用于基因的置换、修正、修饰、失活。
- Results showed that IRES was superior to SV40 promoter for guarantee of genes co expression in bicistronic retrovirus mediated gene therapy. 结果表明:在双基因逆转录病毒载体介导的小鼠骨髓细胞的基因转导中,IRES与内部SV40启动子相比,更能保证双基因的共同表达。
- Retrovirus mediated gene transfection was carried out. First, the package cell line PT67 was transfected by lipofectAMINE TM2000, so as to obtain recombinant retrovirus supernatant, which was directly used in the transfection of MSCs. 反转录病毒介导的基因转染,首先利用LipofectAMINETM 2000转染包装细胞系PT67,获得重组反转录病毒上清,然后用病毒上清直接感染骨髓间充质干细胞。
- METHODS: The eukaryotic expression vector pcDNA3.0 containing human full length HAb18G cDNA and empty vector pcDNA3.0 were introduced respectively into NIH/3T3 fibroblast cells by liposome mediated gene transfection method. 方法 :将含有肝癌相关抗原HAb1 8G全长cDNA的真核表达载体 pcDNA3.;0 /HAb1 8G利用脂质体介导法转染小鼠成纤维细胞NIH/ 3T3;G4 1 8筛选建立稳定表达阳性克隆细胞株t3T3; 利用流式细胞仪、间接免疫荧光染色方法鉴定目的蛋白转染情况 ;
- Study on Rapid, Simple and Very Efficient Method for PCR Mediated Amplification and Labeling of Target Gene in Bacterial Cell 简单、快速、高效扩增和标记目的基因技术的研究
- Lu Deyang,Fan Yunliu,Yu Meimin,Tang Shunxue,Hou Ning,WANG Qing.Regeneration of HNP transgenic alfalfa plants by Agrobacterium mediated gene transrer[J].Acta Genetica Sinic,2000,27(4):331-337. [3]吕德扬;范云六;俞梅敏;唐顺学;侯宁;汪清.;苜蓿高含硫氨基酸蛋白转基因植株再生[J]
- chromosome mediated gene transfer CMGT
- chromosome mediated gene transfer(CMGT) 染色体介导的基因转移
- Objective:To investigate the efficiency of cationic liposome in mediating gene transfer. 目的:研究阳离子脂质体介导体内外基因转移的效率。
- C3d-P28 coding gene was amplified by PCR. 将PCR获得的补体C3d-P28 DNA序列以头尾串连的方式构建四聚体。
- Cloning of S-locus gene by PCR walking in Brassica campestris L. PCR步行法克隆油菜自交不亲和基因。
- Heat inducible FLP recombinase mediated gene excision from transgenic tobacco 热诱导FLP重组酶删除转基因烟草外源基因
- The PCR detection of karnal bunt of wheat. 小麦印度腥黑穗病菌PCR检测。
- Construction of Rate-Limiting Multigene- Cassette in Shikimate Pathway and Gene Replacement in E. Coli 大肠杆菌莽草酸途径限速酶多基因盒的构建及基因替换
