METHODS: The eukaryotic expression vector pcDNA3.0 containing human full length HAb18G cDNA and empty vector pcDNA3.0 were introduced respectively into NIH/3T3 fibroblast cells by liposome mediated gene transfection method.

• 方法 :将含有肝癌相关抗原HAb1 8G全长cDNA的真核表达载体 pcDNA3.；0 /HAb1 8G利用脂质体介导法转染小鼠成纤维细胞NIH/ 3T3；G4 1 8筛选建立稳定表达阳性克隆细胞株t3T3; 利用流式细胞仪、间接免疫荧光染色方法鉴定目的蛋白转染情况 ;