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- The specific primers were designed to amplify the coat protein genes of lily symptomless virus(LSV,genus Carlavirus),lily mottle virus(LMoV,genus Potyvirus) and lily virus X(LVX,genus Potexvirus) respectively. 设计3对表达引物分别扩增侵染了百合无症病毒(lily symptomless virus,LSV),百合斑驳病毒(lily mottle virus,LMoV)和百合X病毒(lily virus X,LVX)的外壳蛋白基因,并在大肠杆菌BL21(DE3)plysS中原核表达。
- The adoptation of PCR-Sequence specific primers (SSP) for HLA-DQB"medium resolution" typing was reported. 采用PCR-序列特异性引物技术(SSP)对HLA-DQB作中分辨法分型。
- Methods Type specific primers and PCR were used to detect the HBV genotypes of 127 Uighur CHB patients in Xinjiang. 方法采用型特异性引物巢式PCR法对127例维吾尔族慢性乙型肝炎患者进行基因分型,并测序验证。
- Two pairs of specific primers: Tch old and Tch new primers were designed based on the result of 18S rRNA gene sequence. 经试验证明这两对引物能够非常有效的区分吕氏泰勒虫和尤氏泰勒虫。
- According to the four types of virulence genes: stx1, stx2, eaeA and hlyA, four pairs of specific primers have been designed. [方法]针对产志贺毒素大肠杆菌(STEC)的stx1、stx2、eaeA、hlyA4种毒力基因,设计了4对特异性引物。
- Genomic DNA of the three yeast strains including TY-1, W1 and W2 were amplified by PCR with specific primers of delta sequence. 提取培养酵母TY-1和野生酵母W1,W2的基因组DNA,进行Delta-PCR,可以分别得到稳定而独特的DNA指纹图谱。
- Methods: Total RNA was extracted from the lung tissue of a new born mouse, and the HMGB1 A box gene was obtained by RT-PCR using specific primers. 方法:从鼠肺提取总RNA,经RT-PCR获得HMGB1 A盒基因。
- Methods:The methods of polymerase chain reaction-sequence specific primers(PCR-SSP) was used to genotype ABO blood group. 方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)基因定型方法对ABO疑难血型进行检测。
- Methods PCR-SSP was set up by synthesized 29 specific primers and 1 pairs of internal control primer,20 PCR reaction for DR alleles. 方法合成29个特异性引物和1对阳性对照引物,组成20个PCR反应用于DR位点,建立一步法PCR-SSP。
- A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence. 根据苯酚羟化酶基因高度保守序列设计一对该基因的特异引物。
- Methods We used a hot initiated PCR based method with asset of universal acanthamoeba specific primers to detect acanthamoeba. 方法以一段特异性通用引物并配合热启动聚合酶链反应技术来检测临床标本中的棘阿米巴原虫。
- A chitinase A gene (chiA) was successfully amplified by PCR using a pair of specific primers from Serratia marcescens Q901, and cloned into pUC19 vector. 用设计的特异性引物和PCR技术,成功地从粘质沙雷氏菌(Serratia marcescens)Q901基因组DNA中扩增并克隆几丁质酶A基因(chiA)序列(GenBank登录号:AY433954)。
- The sequence specific primers of the gene mutation of the mannose combining agglutinator gene exon I at 52 site condon was detected with polymerase chain reaction. 进行甘露糖结合凝集素外显子I52位密码子基因突变的序列特异性引物聚合酶链式反应检测。
- Methods:78 strains of clinically isolated staphylococci were detected by specific primers in conserved regions of mecA,ermA,ermC genes and compared with MIC determination. 方法 :根据上述抗性基因保守区域选用引物经聚合酶链反应检测成都地区临床分离78株耐药葡萄球菌与临床药敏试验比较并追踪其预后。
- Thengene specific primers were designed flanking putative open reading frames (OREs),and three full-length eDNA clones of NJS2, NJS4 and NJS22 genes had beenobtained by PCR. NJS4推测为小麦的一个丝氨酸/苏氨酸蛋白激酶。 NJS22推测为小麦的一个硫代硫酸硫转移酶基因。
- The ORF1 sequence of gene mrp encoding muramidase-released protein(MRP) was amplified from genomic DNA of Streptococcus suis type 2 Qinghai strain by PCR with specific primers. 根据猪链球菌2型溶菌酶释放蛋白基因(mrp)的序列,设计并合成了1对特异性引物,以青海株的基因组DNA为模板扩增了mrp基因ORF1序列。
- Methods Polymerase chain reaction-sequence specific primers (PCR-SSP) were used to determine HLA-A, HLA-B, HLA-DR alleles in 2000 unrelated healthy Han individuals of North China. 方法应用聚合酶链反应-序列特异性引物(PCR-SSP)方法对2000名北方汉族健康志愿者进行A、B、DR等位基因分型。
- This study used PCR method to clone the Tannase gene from Aspergillus oryzae using two specific primers synthesized according to the Tannase gene squence from the genebank. 本研究用PCR 方法从米曲霉（Aspergillus oryzae）ZD中扩增到单宁酶基因编码完整成熟肽的DNA序列，与米曲霉TH的单宁酶基因序列比较，有两个核苷酸不同，从而推测出它们之间有一个氨基酸差异。
- Methods Specific primers were designed to clone the M1, M3 and M5 sequences of astrocyte cells by RT-PCR according to those of neurons,then sequenced the sequences. 方法根据神经细胞M1、M3、M5受体基因序列全长设计特异性探针,采用RT-PCR方法扩增胶质细胞M1、M3、M5受体亚型基因序列,并对其进行克隆测序。
- According to the data of TMV MP in the GeneBank, design gene specific primer, amplified of the whole open reading frame (ORF). 根据GeneBank中TMV MP的序列设计基因特异引物，扩增出运动蛋白基因的整个开放阅读框（ORF）。
