This study used PCR method to clone the Tannase gene from Aspergillus oryzae using two specific primers synthesized according to the Tannase gene squence from the genebank.

• 本研究用PCR 方法从米曲霉（Aspergillus oryzae）ZD中扩增到单宁酶基因编码完整成熟肽的DNA序列，与米曲霉TH的单宁酶基因序列比较，有两个核苷酸不同，从而推测出它们之间有一个氨基酸差异。