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- Construction of the prokaryotic and eukaryotic recombinant plasmid of mip gene of legionella peneumophila 嗜肺军团菌mip基因的原核与真核表达重组质粒的构建
- Construction of eukaryotic recombinant plasmid of herpes simplex virus thymidine kinase gene and its expression in skin tissue of scald rats 单纯疱疹病毒胸苷激酶基因重组真核表达载体的构建及在烫伤大鼠皮肤组织中的表达
- Construction and Expression of Eukaryotic Recombinant Plasmid Including CAV VP3 Gene and Human IL-18 Gene and Influence on Colon Cancer Cell 共表达VP3与hIL-18基因真核重组质粒的构建及对人结肠癌细胞的影响
- Eukaryotic recombinant plasmid 真核重组质粒
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- Objective To construct the recombinant plasmid of eukaryotic expression vector containing M-CSF gene and transfect it into the NIH3T3 cells to study whether resulting in the expression of M-CSF and EGFP fusion proteins. 目的构建真核表达重组体pEGFP-M-CSF,并鉴定其在小鼠成纤维细胞NIH3T3细胞能否正确表达。
- A recombinant expressing plasmid pCMV4/TPO was also constructed by cloning the rhTPO cDNA in an eukaryotic expressing vector pCMV4. When the recombinant plasmid was used to transfect the COS7 cells, temporary expression of rhTPO was detected. 将TPOcDNA亚克隆至哺乳动物细胞瞬时表达载体pCMV4，形成了重组表达质粒pCMV4/TPO。 以该重组质粒转染COS7细胞，可测到TPO在COS7细胞中的瞬时表达
- METHODS: The pcDNA3-E1A recombinant plasmid, designed for high-level expression of E1A gene in a variety of eukaryotic cell lines, was transfected into 686LN-1 cells mediated by lipofectamine? 方法:经脂质体介导,将真核细胞高效表达E1A基因的重组质粒peDNA3-E1A导入人舌鳞癌的淋巴结转移癌细胞系686LN-1。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- This recombinant plasmid then was transfected into the eukaryotic cell COS 7, and the expression of LACK gene in eukaryotic cell was detected by RT PCR and immunofluorescent staining. 将此重组质粒转染 COS- 7细胞 ,通过 RT- PCR及免疫荧光检测 L ACK基因在真核细胞中的表达。
- MethodsThe target gene TspE1 was amplified by RT_PCR, cloned into pUC18 vector, and sub_cloned into the eukaryotic expression vector pcDNA3. BALB/c mice were immunized with the purified recombinant plasmid pcDNA3_TspE1 by gene_gun bombardment. 方法 通过逆转录 聚合酶链反应 (RT PCR)特异性扩增TspE1基因 ,构建重组质粒 pUC18 TspE1,并将TspE1基因亚克隆入真核表达载体 pcDNA3中。
- eukaryotic expression recombinant plasmid 真核表达质粒
- The recombinant plasmid with the hammerhead ribozymegene was correct by digestion identification. 核酶基因重组子经酶切鉴定序列正确。
- Recombinant plasmid pDAlllS was constructed by inserting 1.7kb aprA into the Nrul site of aveD. 将1.;7kb安普霉素抗性基因aprA插入aveD的NruI位点，得pDA1118。
- Study on Expression of Recombinant Plasmid PsecTaq2A-AMG for Human Amelogenin in Human Gingival Fibroblast. 人釉原蛋白重组质粒Psec Taq2A-AMG在人牙龈成纤维细胞表达的研究
- Objective Construct a recombinant plasmid pET28a-EDA-EDB,prepare the fusion EDA-EDB protein. 目的构建重组pET28a-EDA-EDB质粒,制备重组纤维连接蛋白EDA-EDB融合蛋白。
- Objective To investigate the amelogenin expression of recombinant plasmid PcDNA3-AMG in COS-1 cell line. 目的研究人釉原蛋白(AMG)重组质粒PcDNA3-AMG在COS-1细胞系中的表达。
- A recombinant plasmid pUC-IL6 coding for IL6 was constructed by recombinant gene technique. 利用基因重组技术，构建含IL6基因的重组质粒pUC－IL6。
- The recombinant plasmid of antisense TIMP 1 has a certain extent reverse effects on liver fibrosis. 反义TIMP 1表达质粒对肝纤维化有一定的逆转作用
- The recombinant plasmid pBV220-Arr was construted successfully and the target protein Arresten can express in E. 成功构建Arresten基因重组质粒pBV220-Arr;并可在E.
