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- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
- antisense expression vector construction 反义表达载体构建
- Methods An open reading frame of human BSP was amplified by PCR method and was reconstructed into the eukaryotic expressive vector pIRES2-EGFP to construct the hBSP sense or antisense expressive vector . 方法以PCR的方法扩增人BSP的开放阅读框序列,与载体pIRES2-EGFP相连构成重组正反义表达质粒。
- Construction and transient expression of vacuolar invertase gene antisense expression vector in tomato leaves 番茄叶片液泡转化酶基因植物反义表达载体的构建与瞬时表达
- Isolation of a Calmodulin cDNA from Salvia miltiorrhiza Bunge and Construction of its Antisense Expression Vector 丹参钙调蛋白cDNA的克隆及其反义表达载体的构建
- Expression of ethylene receptor gene LeETR2 in tomato mutant Epi and construction of its antisense expression vector 乙烯受体基因LeETR1在番茄Epi和VFN8中的表达及反义表达载体构建(英文)
- Antisense expression vector 反义表达载体
- CLONING OF ACC OXIDASE GENE OF CARNATION AND CONSTRUCTION OF ITS PLANT ANTISENSE EXPRESSION VECTORS 香石竹ACC氧化酶基因克隆及其反义表达载体构建
- Construction of antisense RNA expression vector of the regulation region of hTERT using the adnovirus system by homorecombination in E. Coli. 构建细菌内同源重组型hTERT调控区反义RNA腺病毒载体。
- Mater i a Is and Methods Eukaryotic expression vector of EGFR antisense RNA, pLXSN-AE5? was transfected into SMMC-7721 by using Stearylamine/DOPE (SA liposome). 材料与方法：应用十八酰基胺阳离子脂质体（SA阳离子脂质体）将EGFR反义RNA真核表达载体pLXSN-AE5’导入SMMC-7721细胞中，G418抗性筛选出稳定克隆细胞。
- Methods ECE-1 antisense RNA expression vector with green fluorescent protein as a reporter was constructed and transfected into 16HBE with Fugene 6 TM . 方法 构建ECE -1反义RNA表达载体 ,用脂质体 (FuGENG 6)转染人气道上皮细胞株 (16HBE) ,G418筛选得到稳定表达反义ECE -1RNA的细胞株 (anti -16HBE)。
- Objective To produce antisense vascular endothelial growth factor (VEGF) adenovirus expression vector for the antisense gene therapy of RA with antisense VEGF gene. 目的制备反义血管内皮生长因子(VEGF)腺病毒表达载体,为反义VEGF转基因治疗类风湿关节炎(RA)奠定基础。
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题： 图1.;穿梭表达载体pRL_hEGF的构建。
- Results of PCR amplification and restriction digestion showed that the fragment of antisense gene CYP86MF was introduced into pBI121 plasmid and the expression vector pBI35S-AMF was transferred into Agrobacterium successfully. PCR扩增和酶切鉴定结果表明;所构建的反义CYP86MF基因植物表达载体pBI35S-AMF是正确的;并已成功导入了根癌农杆菌中.
- The efficient expression vector of hKu 70 gene antisense RNA,pEGFP|C1|K has been constructed successfully,it can be used to establish the human cell line which express lower hKu 70 protein and in researches about DSB repair deficiency and toxicology. 成功构建hKu70基因反义RNA真核表达载体pEGFP C1 K ,为建立该基因低表达细胞株、DNA双链断裂修复缺陷和有关毒理学研究提供工具
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- Objective To construct the antisense nucleic acid eukaryotic expressing vector of the cDNA sequence encoding the transmembrane domain of receptor tyrosine kinase RON (RON-RTK) gene (RONm). 目的构建RON基因跨膜区段(RONm)的反义核酸真核表达载体。
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9，将CED-9基因置于CaMV35S启动子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
