Results of PCR amplification and restriction digestion showed that the fragment of antisense gene CYP86MF was introduced into pBI121 plasmid and the expression vector pBI35S-AMF was transferred into Agrobacterium successfully.

• PCR扩增和酶切鉴定结果表明；所构建的反义CYP86MF基因植物表达载体pBI35S-AMF是正确的；并已成功导入了根癌农杆菌中.