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- Keywords interleukin 2;viral fusion protein;China Hamster Oophorus cell;gene expression; 白细胞介素2;病毒融合蛋白;中国仓鼠卵巢细胞;基因表达;
- Viral fusion proteins 病毒融合蛋白质类
- viral fusion protein 病毒融合蛋白
- Purified fusion protein was obtained. 得到纯化的融合蛋白。
- The genes coding for hemagglutinin(H) and fusion protein(F) of measles virus were obtained from viral RNA extracted from supernatant of viral culture by RT PCR,and were cloned into baculovirus transfer vectors,respectively. 采用逆转录－多聚酶链反应（RT－PCR）的方法从麻疹病毒Edmonston株基因组中扩增出血凝素H基因和融合蛋白F基因，并利用转移质粒将这两个基因分别重组到杆状病毒多角体蛋白启动子（PH）控制之下，获得重组病毒vBMVH和vBMVF。
- The GRA7 gene may be expressed as a GST fusion protein in E. coli. GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达。
- These fusion proteins were used to immunize rabbits to raise antisera. 这些表达产物用于免疫动物产生多克隆抗血清。
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF亲和层析柱纯化融合蛋白;
- The fusion protein was purified through Nickel-affinity chromatography column. 用镍柱纯化融合蛋白。
- Methods The recombinant fusion protein MBP hEra was expressed in the E. 方法在大肠杆菌中,表达重组MBP?hEra融合蛋白。
- It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. 它也包括关于包涵体，熔化蛋白质和结晶学的洗净的洗净的章。
- The AML1?ETO fusion proteins generated by chromosome translocation of t (8; 21) lead frequently to pathogenesis of acute myeloid leukemia (AML). t(8;21)染色体易位是导致急性髓系白血病的最常见的发病机制;AML1 ETO是t(8;21)易位所产生的融合基因.
- To obtain the anti-transferrin receptor scFv and streptavidin(SA) recombinant fusion proteins which were high-efficiently and solubly expressed in E. 拟获得在大肠杆菌中能高效可溶表达的转铁蛋白受体单链抗体与链亲和素(SA)的重组融合蛋白。
- Aim:To optimize the fermentation conditions of engineering bacteria expressing fusion proteins composed of aprotinin and to purify aprotinin. 目的:优化融合表达抑肽酶基因工程菌的发酵条件,分离纯化抑肽酶。
- The exprer:,sed fusion proteins was purified by sephmme 6B-amylose affinity chronmtography and DEAE-sepharose Ffchromatography. 表达的融合蛋白 经过Sepharose 6B-amy]ose亲和色谱和DEAE-SephamseFF离子交换色谱得到有效纯化；
- Cyclin D1 and CDK4 expression vectors which can express Cyclin D1 and CDK4 fusion proteins are successfully constructed. 本研究成功构建了细胞周期蛋白D1及CDK4原核表达载体,后两者在细胞内能正确表达细胞周期蛋白D1及CDK4融合蛋白。
- Enterokinase is a serine proteinase extracted from mammal which is widely applied in gene engineering for cleaving fusion proteins. 肠激酶是从哺乳动物体内提取的一种丝氨酸蛋白酶,广泛应用于基因T程领域切割融合蛋白。
- After inductiction ofIPTG,heth of the neurotoxins were overexpres.sed as sohible fusion proteins which were confimed by SDS-PAGE and westemblomng. 表达产物通过SDS-PAGE和蛋白印迹杂交加以鉴定。
- BVP22 can mediate intercellular trafficking of fusion protein in vitro and in vivo. 在体内外BVP22均能够介导与其融合的蛋白在细胞间发生穿梭。
- The study of viruses and viral diseases. 病毒学一种病毒和病毒性疾病的研究
