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- Keywords interleukin 2;viral fusion protein;China Hamster Oophorus cell;gene expression; 白细胞介素2;病毒融合蛋白;中国仓鼠卵巢细胞;基因表达;
- viral fusion protein 病毒融合蛋白
- Purified fusion protein was obtained. 得到纯化的融合蛋白。
- Viral fusion proteins 病毒融合蛋白质类
- The genes coding for hemagglutinin(H) and fusion protein(F) of measles virus were obtained from viral RNA extracted from supernatant of viral culture by RT PCR,and were cloned into baculovirus transfer vectors,respectively. 采用逆转录-多聚酶链反应(RT-PCR)的方法从麻疹病毒Edmonston株基因组中扩增出血凝素H基因和融合蛋白F基因,并利用转移质粒将这两个基因分别重组到杆状病毒多角体蛋白启动子(PH)控制之下,获得重组病毒vBMVH和vBMVF。
- The GRA7 gene may be expressed as a GST fusion protein in E. coli. GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达。
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF亲和层析柱纯化融合蛋白;
- The fusion protein was purified through Nickel-affinity chromatography column. 用镍柱纯化融合蛋白。
- Methods The recombinant fusion protein MBP hEra was expressed in the E. 方法在大肠杆菌中,表达重组MBP?hEra融合蛋白。
- BVP22 can mediate intercellular trafficking of fusion protein in vitro and in vivo. 在体内外BVP22均能够介导与其融合的蛋白在细胞间发生穿梭。
- The titer of antiserum was detected by double agar immunodiffusion with GST-PI fusion protein. 并用双向免疫扩散法鉴定家兔多价抗血清的效价。
- EGF-Ang fusion protein had definitely cytotoxicity to Hep2 cells which over expressed EGFR in vitro. 融合蛋白EGF-Ang在体外对过度表达EGFR的Hep2细胞具有明显的杀伤作用。
- Recombinant TrxA/PEB1 fusion protein could be a valuable candidate for CJ vaccine with great applied value. (4)PEB1融合蛋白作为CJ的亚单位侯选基因工程疫苗不仅可行,而且有良好应用前景。
- Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.coli. 结果:成功克隆了CAT的基因片段,并在大肠杆菌中得到其融合蛋白的表达。
- A novel fusion protein namely IL- 2-Ang consisting of interleukin-2 ( IL-2 ) and angiogenin ( Ang) was expressed in E. 利用基因工程技术在大肠杆菌表达成功人白细胞介素2(IL-2)和人血管生成素(Angiogenin)的融合蛋白(IL-2-Ang)。
- The s-lap/GFP fusion protein can express in Hela cells and locate in entire cells, but express highly in the nucleus. s- lap/ GFP融合蛋白可在人 Hela细胞中表达 ,并主要定位于细胞核。
- Western blot proved that the recombinant protein is the fusion protein of APE/Ref|1. Western印迹证明重组蛋白为APE ref 1融合蛋白 .
- Western-blot analysis with sera against CAV showed the fusion protein had good specificity. 在Western-blot免疫印迹分析中,3个融合蛋白与CAV阳性血清均能发生特异性反应,表明它们都具有良好的反应性。
- These fusion proteins were used to immunize rabbits to raise antisera. 这些表达产物用于免疫动物产生多克隆抗血清。
- The expression vector of PVS CP gene is constructed, a 58kDa fusion protein product is expressed in E. 构建了含PVS CP基因的融合蛋白原核表达载体,并在大肠杆菌(E.;coli
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