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- It is concordant with routine PCR method. 并与常规PCR法具有很好的一致性。
- The ISOObp promoter of PNZIP gene was cloned by adaptor PCR method. 因此,我们利用接头PCR技术克隆了PNZIP基因的启动子。
- The applications of Mullis' PCR method are already many. 原理简介:PCR技术的基本原理是DNA的半保留复制。
- A multiplex- PCR method for detection bovine materials in foodstuffs. 动物饲料中牛成分的复合PCR检测方法。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- Meanwhile, the PCR method is superior to immunohistochemistry in detecting HPV. 在检测上,PCR方法优于免疫组织化学染色,值得提倡和推广。
- Using PCR method, BBTV or CMV can be detected from the banana leaf tissue equivalent to 100 ng. 用PCR方法可从相当于100ng的叶片组织中检测到BBTV和CMV。
- Objective To develop a PCR method for the determination of porcine parvovirus(PPV). 目的建立猪细小病毒(PPV)PCR检测方法。
- The results prove that adapter ligation PCR method is workable to clone carnation CHS promoter. 然后利用衔接头PCR的方法进行了香石竹CHS启动子克隆的初步研究,得到了约500bp和800bp的PCR产物,目前克隆测序工作正在进行。
- This is to develop a rapid,sensitive and specific fluorescence PCR method detecting listeria monocytogenes in food. 发展一种快速、敏感、特异的荧光PCR方法检测食品中单核细胞增生李斯特氏菌。
- In order to improve diagmostic procedure of E. suis, the PCR method was established. 鉴于上述原因,为了建立特异准确的诊断方法,本研究开展了附红细胞体病的聚合酶链式反应(PCR)诊断方法的研究。
- The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- PCR method was considered as the gold standard, which showed the highest specificity (100%) and sensitivity (100%). PCR法是检测MRSA的金标准,特异性和灵敏度均为100%25。
- The NDI gene in mtDNA was amplified with regular PCR method, and the PCR product was sequenced after purified. 用PCR扩增人线粒体NDI基因片段,PCR产物经纯化后测序和核苷酸同源性分析。
- Conclusions PCR method in which 139-141 as primer is suitable for rapid detection of Salmonella. 结论在沙门菌的快速检测中,宜采用以139-141为引物的PCR检测法。
- Objective:This study was to establish a immuno in situ PCR method for the diagnosis of HCMV active infection. 目的:建立用于诊断HCMV活动性感染的原位免疫PCR方法。
- Conclusion The fluorogenic Quantitative PCR method for the detection of Plasmodium falciparum is simple and accurate. 结论 建立了检测恶性疟原虫的荧光定量PCR方法,较常规PCR技术更为简便、快速、准确,有很好的应用前景和研究价值。
- According to the sequence of GAPDH gene available in GenBank,a SYBR Green I dye based on real-time PCR method is developed. 根据GenBank中鸭GAPDH基因的序列设计引物;建立了基于SYBR Green I染料技术的real-time PCR方法.
- All of the 51 Bt were analyzed by the PCR method with general primers of cry1, cry2, cry3, cry4, cry-n, cry7, cry8, cry11, cry13 and cyt genes. 对51 株Bt 分离菌株的cry 基因进行了PCR 鉴定。
- Six truncated Cryllel proteins were induced in Ecoli BL21(DE3) containing six deleted fragments from cryllel gene by PCR method. 本研究通过PCR扩增的方法,以cry1Ie1全长基因为模板,分别得到不同末端缺失的基因片段,转化大肠杆菌BL21(DE3)中,诱导表达,得到6种末端缺失的蛋白。