Six truncated Cryllel proteins were induced in Ecoli BL21(DE3) containing six deleted fragments from cryllel gene by PCR method.

 
  • 本研究通过PCR扩增的方法,以cry1Ie1全长基因为模板,分别得到不同末端缺失的基因片段,转化大肠杆菌BL21(DE3)中,诱导表达,得到6种末端缺失的蛋白。
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