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- Methods Using the 3H TdR and 125 I UdR methods. 方法 采用3H TdR掺入法和12 5I UdR释放法 .
- METHODS Techniques of cell culture, MTT test, 3H TdR incorporation and flow cytometric DNA analysis were used. 方法 应用离体培养的PASMC;采用四唑盐 (MTT)比色法实验、3H-胸腺嘧啶核苷(3H- Td R)参入和流式细胞仪技术 ;观察 PASMC的增殖、DNA合成和细胞周期的变化 .
- In simple hypoxia condition, HSF in G 0/G 1 phase were increased, but 3H TdR uptake was decreased. 单纯缺氧可使HSFG0/G1期数目增多,3HTdR摄取量降低;
- Methods: The morphology of HSF was observed under an electron microscope. The cell cycle was analyzed with flow cytometry. 3H TdR uptake was tested with isotope labeled method. 方法电镜观察细胞形态,流式细胞仪测定细胞周期,同位素放射测定3H胸腺嘧啶核苷(3HTdR)摄取量。
- On the basis of established nude mice transplanted tumor model of human tongue squamous cell carcinoma, peritumor injection was performed with 3H TdR labeled TIL from human tongue cancer after 4 weeks culture. 用3H-胸腺嘧啶核苷(3H-TdR)标记体外培养4周的人舌癌肿瘤浸润淋巴细胞(TIL)。
- The expression of p16 protein was observed. The changes of the proliferation and DNA synthesis of KFb before and after transfection were observed and compared by drafting cell growth curve and by 3H TdR incorporation method. 随后对已转染及未转染的KFb进行免疫细胞化学染色 ,观察p16蛋白的表达 ,并通过绘制细胞生长曲线及采用氚标记胸腺嘧啶脱氧核苷 (3 H TdR)掺入法 ,比较转染前后细胞在生长增殖及DNA合成代谢方面的变化。
- Methods APC:T:B cell reactive system was established in vitro. T cell proliferation was analyzed by using 3H TdR incorporation in the blockade of B7/CD28 with B7 1mAb and CsA. B cell antibody production was assayed by using ELISA. 方法 在体外建立抗原递呈细胞 T淋巴细胞 B淋巴细胞反应系统 ,利用环孢素A(CsA)与抗B7 1单克隆抗体联用阻断B7:CD2 8共刺激途径 ,采用3H TdR法检测T淋巴细胞增殖 ,酶联免疫吸附试验 (ELISA法 )测定B淋巴细胞分泌的抗体。
- Methods The lymphocytes and subsets of cord blood were separated and cultured. DNA synthesis and K 562 cell activity were measured by 3H TdR,the hematopoietic ability of stem cells was observed by colony culture. 方法 对脐血T淋巴细胞及其亚群分离、培养 ,LDR后用3 H 胸腺嘧啶核苷 (3 H TdR)掺入法观察其DNA合成和抗肿瘤细胞K562 活性 ,集落培养观察脐血干细胞造血能力。
- Methods: Anti HBs antibodies were measured by ELISA titers ,and lymphocyte proliferation was detected through 3H TdR incorporation. Humoral and cellu lar responses of different groups were then compared. 方法 :以ELISA法检测免疫小鼠血清抗HBs抗体 ,另用3 H TdR掺入法测定淋巴细胞增殖活性 ,初步研究不同组的体液和细胞免疫应答。
- DNA and protein synthesis was detected by [3H]thymidine as well as [3H]TdR incorporation,respectively. [3H]亮氨酸掺入法及[3H]胸腺嘧啶核苷掺入法检测细胞内蛋白、DNA合成。
- tritiated thymidine(3H-TdR) 氚-胸腺嘧啶核苷, 氚标记胸苷
- tritiated thymidine 氚标记胸腺嘧啶核苷
- Comparative Study of MTT Colorimetric Assay and 3H TdR Incorporation Assay on the Effects of MLC in Swine MTT比色法与~3H-TdR掺入法测定猪MLC效果的比较研究
- 3H TdR was added 18 h before the end of culture. Immune response and the antigen presenting action of Kupffer cells were analysis by lymphocyte transformation test (LTT). 终止培养前 18h加入氚标记胸腺嘧啶核苷 (3H- Td R) ,通过淋巴细胞转化试验 (L TT)分析机体吸入氟烷后诱导免疫应答过程中 Kupffer细胞的抗原递呈作用。
- We have studied several factors affecting the uptake of [~3H] thymidine ([~3H]TdR) by the fresh spleen nucleated cells isolated from C57BL mice. 本文以测定氚化胸腺嘧啶核苷(~3H-TdR)掺入新鲜制备的离体的小鼠脾有核细胞DNA的方法;来判断细胞DNA合成能力;并对影响脾细胞DNA合成的某些基本因素的作用进行了研究。 研究表明;2毫升pH7.;4的Eagle培养液中含4×10(?)
- methyl 3H TdR 甲基-3H-TdR
- By using cell counting and thymidine ( 3 H TdR) up take method, the effects of ligustrazine on the proliferation of aortic SMC from 4 rabbits were also examined. 利用细胞计数和氚-胸腺嘧啶脱氧核苷(3H-TdR)掺入方法观察川芎嗪对离体培养的4只兔(4月龄)动脉SMC增殖的影响。
- Tritium labeled thymine deoxyriboside ([ 3H] TdR) uptake was measured to observe DNA synthesis. 氚胸腺嘧啶核苷([3H]TdR)掺入法检测TP40对T24细胞蛋白质合成的抑制作用。
- LPA enhanced the cultured VSMC?3H?TdR incorporation in a concentration?dependent manner, and increased MAPK activity concurrently. LPA呈浓度依赖地刺激3H?TdR参入,并平行地激活MAPK,二者间呈显著正相关。
- METHODS: The synthesis of DNA and collagen was determined by measuring the incorporation of [3H]TdR and [3H]proline of HLF respectively. 方法:采用[~3H]TdR与[~3H]脯氨酸掺入分别测定脱氧核糖核酸和胶原合成。