METHODS Techniques of cell culture, MTT test, 3H TdR incorporation and flow cytometric DNA analysis were used.

• 方法 应用离体培养的PASMC；采用四唑盐 (MTT)比色法实验、3H-胸腺嘧啶核苷(3H- Td R)参入和流式细胞仪技术 ；观察 PASMC的增殖、DNA合成和细胞周期的变化 .